MK-8527 is a novel inhibitor of HIV-1 reverse transcriptase translocation with potential for extended-duration dosing
Fig 8
HIV-RT primer extension assay [23] to evaluate the chain termination mechanism of MK-8527-TP compared with ISL-TP and ddATP (an NRTI).
(A) Primer and template used along with representative gel with DCT and ICT sites marked. Dose-dependent chain termination patterns of (B) ddATP, (C) ISL-TP, and (D) MK-8527-TP are shown. The original uncropped blots can be found in S2 Raw Images. DCT, delayed chain termination; ddATP, dideoxyadenosine triphosphate; ICT, immediate chain termination; ISL-TP, islatravir-triphosphate; NRTI; nucleos(t)ide reverse transcriptase inhibitor, RT, reverse transcriptase; TP, triphosphate.