PLOS Pathogens

In Gram-negative bacteria, multi-subunit efflux pumps transport molecules across the inner membrane, periplasm, and outer membrane to the extracellular environment. These efflux pumps include the Resistance-Nodulation-Cell Division (RND) superfamily, which utilize the membrane proton motive force to export a wide range of substrates against a concentration gradient. 

Orthoflaviviruses, including Dengue, West Nile and ZIKV, cause significant global disease yet lack broadly effective antiviral drugs. To address this unmet need, we used a cell-based high-content screening approach to discover a novel class of small molecules that inhibit a previously underexplored viral protein, NS2A. 

The single mitochondrion in the liver and asexual blood stages of malaria parasites multiplying in the human host supports parasite growth and multiplication. It adopts complex branched morphology in these stages, but mitochondrial biogenesis through the parasite life cycle is not well understood.