MK-8527 is a novel inhibitor of HIV-1 reverse transcriptase translocation with potential for extended-duration dosing
Fig 7
Iron footprinting assay [24] to evaluate the position of RT on the primer and template.
An iron footprinting assay (A) was performed in the presence of (B) 5 µM ddATP; (C) 1 µM ISL-TP; or (D) µM MK-8527-TP; and escalating concentrations of dTTP. The original uncropped blots can be found in S1 Raw Images. DCT, delayed chain termination; ddATP, dideoxyadenosine triphosphate; dTTP, deoxythymidine triphosphate; ICT, immediate chain termination; ISL, islatravir; MP, monophosphate; RT, reverse transcriptase; TP, triphosphate.