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A Species-Specific Cluster of Defensin-Like Genes Encodes Diffusible Pollen Tube Attractants in Arabidopsis

Figure 2

Phylogenetic and syntenic relationship between the CRP810_1 genes of A. thaliana and A. lyrata.

(A) Phylogenetic tree of the CRP810_1 (blue) and AlCRP810_1 (red) genes based on the coding region of their genomic sequences, with At4g08869 and At4g08875 (the closest related genes to CRP810_1 genes) as the outgroup. Only bootstrap values ≥90 are indicated. The scale shows the number of substitutions per site. (B) Representations of syntenic regions containing CRP810_1 and AlCRP810_1 genes in the A. thaliana (Col-0) and A. lyrata genomes. Blue and red arrows represent CRP810_1 and AlCRP810_1 genes, respectively. Gray arrows show flanking genes. Dashed lines indicate syntenic genes between A. thaliana and A. lyrata. The genes with asterisks in (A and B) are probably nonfunctional. Also see Figure S1. (C) Full-length amino acid sequences of CRP810_1.1 to _1.5 and CRP810_1.6 (Ψ). The arrow indicates the position of the predicted cleavage sites. Asterisks mark conserved cysteine residues. Also see Figure S2 for comparison with AlCRP810_1.

Figure 2

doi: https://doi.org/10.1371/journal.pbio.1001449.g002