July 2022

Immunofluorescence image of an E11.0 Tmem2-FLAG^KI knock-in reporter embryo stained for TMEM2 (with anti-FLAG antibody, green) and hyaluronan (with biotinylated hyaluronan-binding protein, red). Nuclei were counterstained with DAPI (Blue). TMEM2 is strongly expressed in tissues populated by neural crest derivatives, including the facial prominence, the branchial arches, the dorsal root ganglia, and the developing heart. Double-staining for TMEM2 and hyaluronan reveals that TMEM2 and hyaluronan exhibit roughly inverse patterns of distribution, consistent with the notion that matrix-associated HA is removed from sites where TMEM2 is actively expressed. See Inubushi et al.

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Image Credit: Toshihiro Inubushi

Viewpoints

Double take: A dual-functional Hypr GGDEF synthesizes both cyclic di-GMP and cyclic GMP—AMP to control predation in Bdellovibrio bacteriovorus

Aathmaja Anandhi Rangarajan, Christopher M. Waters

PLOS Genetics: published July 21, 2022 | https://doi.org/10.1371/journal.pgen.1010263

Research Articles

“The ubiquitin ligase SIAH2 is a female-specific regulator of circadian rhythms and metabolism”

Tsedey Mekbib, Ting-Chung Suen, Aisha Rollins-Hairston, Kiandra Smith, Ariel Armstrong, Cloe Gray, Sharon Owino, Kenkichi Baba, Julie E. Baggs, J. Christopher Ehlen, Gianluca Tosini, Jason P. DeBruyne

PLOS Genetics: published July 5, 2022 | https://doi.org/10.1371/journal.pgen.1010305

Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency

Lei Luo, Yan Shi, Huanan Wang, Zizengchen Wang, Yanna Dang, Shuang Li, Shaohua Wang, Kun Zhang

PLOS Genetics: published July 5, 2022 | https://doi.org/10.1371/journal.pgen.1010307

A locus at 19q13.31 significantly reduces the ApoE ε4 risk for Alzheimer’s Disease in African Ancestry

Farid Rajabli, Gary W. Beecham, Hugh C. Hendrie, Olusegun Baiyewu, Adesola Ogunniyi, Sujuan Gao, Nicholas A. Kushch, Marina Lipkin-Vasquez, Kara L. Hamilton-Nelson, Juan I. Young, Derek M. Dykxhoorn, Karen Nuytemans, Brian W. Kunkle, Liyong Wang, Fulai Jin, Xiaoxiao Liu, Briseida E. Feliciano-Astacio, Alzheimer’s Disease Sequencing Project, Alzheimer’s Disease Genetic Consortium, Gerard D. Schellenberg, Clifton L. Dalgard, Anthony J. Griswold, Goldie S. Byrd, Christiane Reitz, Michael L. Cuccaro, Jonathan L. Haines, Margaret A. Pericak-Vance, Jeffery M. Vance

PLOS Genetics: published July 5, 2022 | https://doi.org/10.1371/journal.pgen.1009977

Fragmentomics of urinary cell-free DNA in nuclease knockout mouse models

Meihui Chen, Rebecca W. Y. Chan, Peter P. H. Cheung, Meng Ni, Danny K. L. Wong, Ze Zhou, Mary-Jane L. Ma, Liangbo Huang, Xinzhou Xu, Wing-Shan Lee, Guangya Wang, Kathy O. Lui, W. K. Jacky Lam, Jeremy Y. C. Teoh, Chi-Fai Ng, Peiyong Jiang, K. C. Allen Chan, Rossa W. K. Chiu, Y. M. Dennis Lo

PLOS Genetics: published July 6, 2022 | https://doi.org/10.1371/journal.pgen.1010262

Dioecy and chromosomal sex determination are maintained through allopolyploid speciation in the plant genus Mercurialis

Melissa A. Toups, Beatriz Vicoso, John R. Pannell

PLOS Genetics: published July 6, 2022 | https://doi.org/10.1371/journal.pgen.1010226

Distinct functions of three chromatin remodelers in activator binding and preinitiation complex assembly

Yashpal Rawal, Hongfang Qiu, Alan G. Hinnebusch

PLOS Genetics: published July 6, 2022 | https://doi.org/10.1371/journal.pgen.1010277

Evaluating indirect genetic effects of siblings using singletons

Laurence J. Howe, David M. Evans, Gibran Hemani, George Davey Smith, Neil M. Davies

PLOS Genetics: published July 7, 2022 | https://doi.org/10.1371/journal.pgen.1010247

Periplasmic oxidized-protein repair during copper stress in E. coli: A focus on the metallochaperone CusF

Alexandra Vergnes, Camille Henry, Gaia Grassini, Laurent Loiseau, Sara El Hajj, Yann Denis, Anne Galinier, Didier Vertommen, Laurent Aussel, Benjamin Ezraty

PLOS Genetics: published July 11, 2022 | https://doi.org/10.1371/journal.pgen.1010180

Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella

Daofeng Qu, Mengxue Jiang, Calder Duffin, Kelly T. Hughes, Fabienne F. V. Chevance

PLOS Genetics: published July 12, 2022 | https://doi.org/10.1371/journal.pgen.1010313

SlJAZ10 and SlJAZ11 mediate dark-induced leaf senescence and regeneration

Boyan Tang, Tingting Tan, Yating Chen, Zongli Hu, Qiaoli Xie, Xiaohui Yu, Guoping Chen

PLOS Genetics: published July 13, 2022 | https://doi.org/10.1371/journal.pgen.1010285

The meiotic cohesin subunit REC8 contributes to multigenic adaptive evolution of autopolyploid meiosis in Arabidopsis arenosa

Chris Morgan, Emilie Knight, Kirsten Bomblies

PLOS Genetics: published July 13, 2022 | https://doi.org/10.1371/journal.pgen.1010304

Polygenic and socioeconomic risk for high body mass index: 69 years of follow-up across life

David Bann, Liam Wright, Rebecca Hardy, Dylan M. Williams, Neil M. Davies

PLOS Genetics: published July 14, 2022 | https://doi.org/10.1371/journal.pgen.1010233

The evolutionary path of chemosensory and flagellar macromolecular machines in Campylobacterota

Ran Mo, Siqi Zhu, Yuanyuan Chen, Yuqian Li, Yugeng Liu, Beile Gao

PLOS Genetics: published July 14, 2022 | https://doi.org/10.1371/journal.pgen.1010316

Estimating the timing of multiple admixture events using 3-locus linkage disequilibrium

Mason Liang, Mikhail Shishkin, Anastasia Mikhailova, Vladimir Shchur, Rasmus Nielsen

PLOS Genetics: published July 15, 2022 | https://doi.org/10.1371/journal.pgen.1010281

The cell surface hyaluronidase TMEM2 plays an essential role in mouse neural crest cell development and survival

Toshihiro Inubushi, Yuichiro Nakanishi, Makoto Abe, Yoshifumi Takahata, Riko Nishimura, Hiroshi Kurosaka, Fumitoshi Irie, Takashi Yamashiro, Yu Yamaguchi

PLOS Genetics: published July 15, 2022 | https://doi.org/10.1371/journal.pgen.1009765

Estimation of causal effects of a time-varying exposure at multiple time points through multivariable mendelian randomization

Eleanor Sanderson, Tom G. Richardson, Tim T. Morris, Kate Tilling, George Davey Smith

PLOS Genetics: published July 18, 2022 | https://doi.org/10.1371/journal.pgen.1010290

Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex

Hong Sun, Zhenfang Wu, Yuanze Zhou, Yanjia Lu, Huaisheng Lu, Hongwen Chen, Shaohua Shi, Zhixiong Zeng, Jian Wu, Ming Lei

PLOS Genetics: published July 18, 2022 | https://doi.org/10.1371/journal.pgen.1010308

HAP40 is a conserved central regulator of Huntingtin and a potential modulator of Huntington’s disease pathogenesis

Shiyu Xu, Gang Li, Xin Ye, Dongsheng Chen, Zhihua Chen, Zhen Xu, Moretti Daniele, Sara Tambone, Alessandra Ceccacci, Licia Tomei, Lili Ye, Yue Yu, Amanda Solbach, Stephen M. Farmer, Erin Furr Stimming, George McAllister, Deanna M. Marchionini, Sheng Zhang

PLOS Genetics: published July 19, 2022 | https://doi.org/10.1371/journal.pgen.1010302

Differentiated function and localisation of SPO11-1 and PRD3 on the chromosome axis during meiotic DSB formation in Arabidopsis thaliana

Christophe Lambing, Pallas Kuo, Jaeil Kim, Kim Osman, Amy Leanne Whitbread, Jianhua Yang, Kyuha Choi, F. Chris H. Franklin, Ian R. Henderson

PLOS Genetics: published July 20, 2022 | https://doi.org/10.1371/journal.pgen.1010298

Multi-color dSTORM microscopy in Hormad1^-/^- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structure

Lieke Koornneef, Johan A. Slotman, Esther Sleddens-Linkels, Wiggert A. van Cappellen, Marco Barchi, Attila Tóth, Joost Gribnau, Adriaan B. Houtsmuller, Willy M. Baarends

PLOS Genetics: published July 20, 2022 | https://doi.org/10.1371/journal.pgen.1010046

Combinatorial Gli activity directs immune infiltration and tumor growth in pancreatic cancer

Michael K. Scales, Ashley Velez-Delgado, Nina G. Steele, Hannah E. Schrader, Anna M. Stabnick, Wei Yan, Nayanna M. Mercado Soto, Zeribe C. Nwosu, Craig Johnson, Yaqing Zhang, Daniel J. Salas-Escabillas, Rosa E. Menjivar, H. Carlo Maurer, Howard C. Crawford, Filip Bednar, Kenneth P. Olive, Marina Pasca di Magliano, Benjamin L. Allen

PLOS Genetics: published July 22, 2022 | https://doi.org/10.1371/journal.pgen.1010315

Synaptic components are required for glioblastoma progression in Drosophila

María Losada-Pérez, Mamen Hernández García-Moreno, Irene García-Ricote, Sergio Casas-Tintó

PLOS Genetics: published July 25, 2022 | https://doi.org/10.1371/journal.pgen.1010329

Cytidinediphosphate diacylglycerol synthase—Mediated phosphatidic acid metabolism is crucial for early embryonic development of Arabidopsis

Xin-Qiao Du, Hong-Yan Yao, Pan Luo, Xing-Chun Tang, Hong-Wei Xue

PLOS Genetics: published July 25, 2022 | https://doi.org/10.1371/journal.pgen.1010320

Motor defects in a Drosophila model for spinal muscular atrophy result from SMN depletion during early neurogenesis

Stuart J. Grice, Ji-Long Liu

PLOS Genetics: published July 25, 2022 | https://doi.org/10.1371/journal.pgen.1010325

Dispersive forces and resisting spot welds by alternative homolog conjunction govern chromosome shape in Drosophila spermatocytes during prophase I

Luisa Vernizzi, Christian F. Lehner

PLOS Genetics: published July 27, 2022 | https://doi.org/10.1371/journal.pgen.1010327

Repression by the H-NS/YmoA histone-like protein complex enables IscR dependent regulation of the Yersinia T3SS

David Balderas, Mané Ohanyan, Pablo A. Alvarez, Erin Mettert, Natasha Tanner, Patricia J. Kiley, Victoria Auerbuch

PLOS Genetics: published July 28, 2022 | https://doi.org/10.1371/journal.pgen.1010321

Initiator tRNA lacking 1-methyladenosine is targeted by the rapid tRNA decay pathway in evolutionarily distant yeast species

Monika Tasak, Eric M. Phizicky

PLOS Genetics: published July 28, 2022 | https://doi.org/10.1371/journal.pgen.1010215

The RNA-binding protein Puf5 and the HMGB protein Ixr1 contribute to cell cycle progression through the regulation of cell cycle-specific expression of CLB1 in Saccharomyces cerevisiae

Megumi Sato, Kaoru Irie, Yasuyuki Suda, Tomoaki Mizuno, Kenji Irie

PLOS Genetics: published July 29, 2022 | https://doi.org/10.1371/journal.pgen.1010340

Methods

Enrichment of centromeric DNA from human cells

Riccardo Gamba, Giulia Mazzucco, Therese Wilhelm, Leonid Velikovsky, Catalina Salinas-Luypaert, Florian Chardon, Julien Picotto, Mylène Bohec, Sylvain Baulande, Ylli Doksani, Daniele Fachinetti

PLOS Genetics: published July 19, 2022 | https://doi.org/10.1371/journal.pgen.1010306

Fine-mapping from summary data with the “Sum of Single Effects” model

Yuxin Zou, Peter Carbonetto, Gao Wang, Matthew Stephens

PLOS Genetics: published July 19, 2022 | https://doi.org/10.1371/journal.pgen.1010299

Cover

The cell surface hyaluronidase TMEM2 plays an essential role in mouse neural crest cell development and survival.