The Integrator complex regulates differential snRNA processing and fate of adult stem cells in the highly regenerative planarian Schmidtea mediterranea
Fig 7
ints RNAi reduces differentiated cell output.
EdU pulse-chase labeling combined with FISH staining of differentiation markers. 10 days following the first dsRNA injection, animals were pulsed with EdU, fixed 3, 7 or 9 days later and cell type markers were visualized by FISH. (A) Quantification of EdU-positive cells expressing the indicated marker, normalized to the total number of EdU-positive cells. Chase periods were 3 days for NB.21.11.e, myoD and gata4/5/6, 7 days for AGAT-1 and 9 days for porcupine, pc2, zpuf-6 and collagen. Circles represent measurements of individual animals. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. (B, C, D) Representative images of EdU-labelled cells expressing the intestinal (porcupine), neuronal (pc2) or epidermal progeny (zpuf-6) marker. Overview images and insets are single confocal sections acquired with a 20x and a 63x objective, respectively. Arrowheads indicate marker+EdU+ cells. Scale bars: overview pictures = 50 μm, magnifications = 5 μm.