In multicellular organisms, cell type diversity and fate depend on specific sets of transcript isoforms generated by post-transcriptional RNA processing. Here, we used Schmidtea mediterranea, a flatworm with extraordinary regenerative abilities and a large pool of adult stem cells, as an in vivo model to study the role of Uridyl-rich small nuclear RNAs (UsnRNAs), which participate in multiple RNA processing reactions including splicing, in stem cell regulation. We characterized the planarian UsnRNA repertoire, identified stem cell-enriched variants and obtained strong evidence for an increased rate of UsnRNA 3’-processing in stem cells compared to their differentiated counterparts. Consistently, components of the Integrator complex showed stem cell-enriched expression and their depletion by RNAi disrupted UsnRNA processing resulting in global changes of splicing patterns and reduced processing of histone mRNAs. Interestingly, loss of Integrator complex function disrupted both stem cell maintenance and regeneration of tissues. Our data show that the function of the Integrator complex in UsnRNA 3’-processing is conserved in planarians and essential for maintaining their stem cell pool. We propose that cell type-specific modulation of UsnRNA composition and maturation contributes to in vivo cell fate choices, such as stem cell self-renewal in planarians.
Stem cells not only give rise to all specialized cell types of the body through differentiation but also to new stem cells through the process of self-renewal. Modifications of messenger RNAs (mRNAs) following their transcription play an important role in a stem cell’s decision between self-renewal and differentiation. However, since these cells are rare and difficult to access in mammals, the underlying mechanisms have mostly been studied in cultured cells. Here, we employ the planarian flatworm Schmidtea mediterranea that maintains a large pool of highly potent stem cells throughout life as an in vivo model to analyze the function of UsnRNAs, important regulators of multiple mRNA processing steps, in stem cell regulation. We show that stem cells express more UsnRNA sequence variants compared to differentiated cells. Moreover, the activity of the Integrator complex, which mediates UsnRNA maturation, is increased in stem cells. Depletion of Integrator complex components disrupts their global splicing pattern and affects the processing of histone mRNAs resulting in the loss of stem cell self-renewal and, consequently, in defects of tissue homeostasis and regeneration. Thus, we propose that planarian stem cell self-renewal in vivo depends on differential variant expression and efficient processing of UsnRNAs.
Citation: Schmidt D, Reuter H, Hüttner K, Ruhe L, Rabert F, Seebeck F, et al. (2018) The Integrator complex regulates differential snRNA processing and fate of adult stem cells in the highly regenerative planarian Schmidtea mediterranea. PLoS Genet 14(12): e1007828. https://doi.org/10.1371/journal.pgen.1007828
Editor: A. Aziz Aboobaker, University of Oxford, UNITED KINGDOM
Received: September 6, 2017; Accepted: November 12, 2018; Published: December 17, 2018
Copyright: © 2018 Schmidt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: Next generation sequencing data is available under Bioproject PRJNA397855: SAMN07490271 (X1_gfp_RNAi), SAMN07490272 (X1_ints3_RNAi), SAMN07490273 (X1_ints9_RNAi), SAMN07490274 (small RNAs_wt_intact). Partial coding sequences of ints were deposited in Genbank: MF671814 (ints3) and MF671815 (ints9).
Funding: This work was supported by the Max-Planck-Gesellschaft, the Deutsche Forschungsgemeinschaft (German Research Foundation) (BA4044/3-1) and the European Research Council (ERC-2016-StG 716894-IniReg). MI was supported by the European Research Council under the European Union's Horizon 2020 program (ERC-StG-LS2-637591), the Spanish Ministerio de Economía y Competitividad (BFU2017-89201-P) and the ‘Centro de Excelencia Severo Ochoa 2013-2017’(SEV-2012-0208). We acknowledge the support of the CERCA Programme/Generalitat de Catalunya and of the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the EMBL partnership. The Bartscherer lab is part of the Cells-in-Motion Cluster of Excellence (EXC 1003-CiM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The cell types composing a multicellular organism differ significantly in morphology and function, despite their identical genetic information. These features are determined in a differentiation process starting from a common progenitor, a stem cell, through a number of cell fate decisions. While cell fate can be influenced by regulation at every step of gene expression as well as of function and turnover of the gene products, the role of transcriptional changes has been investigated in greatest detail. The regulation of pluripotent stem cell fate, in particular, has been attributed to networks of transcription factors and chromatin modifiers [1–3]. However, recent studies identified key functions of post-transcriptional RNA processing in stem cell self-renewal and fate regulation including alternative splicing (AS) [4–7] and -polyadenylation  as well as chemical nucleotide modifications . Intriguingly, core components of the spliceosome, including Uridyl-rich small nuclear RNAs (UsnRNAs), were recently implicated in the regulation of pluripotency [10,11]. The studies uncovering these mechanisms employed mammalian embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in vitro. However, comparable cell types are present in vivo only during a short period of development or are exceedingly rare. The role of these factors in vivo remains, thus, largely obscure.
In contrast to mammals, some invertebrates maintain highly potent stem cells lifelong. The stem cells of the planarian S. mediterranea, collectively termed neoblasts, constitute around 25% of all adult body cells and a substantial fraction of them are pluripotent . This abundance of adult stem cells is the basis of the outstanding regenerative abilities and tissue plasticity of adult planarians (reviewed in ). The ability to modify neoblast dynamics by tissue amputation or feeding and to transplant them between individual animals, allowing full repopulation of a neoblast-depleted animal from a single donor neoblast, together with their amenability to RNA interference (RNAi) make planarians a powerful model organism for studying stem cell regulation in vivo. In a recent study, we identified distinct AS patterns of planarian neoblasts and of their differentiated progeny and showed that they are shaped by the RNA binding protein bruli, a CELF family member, and its antagonists of the MBNL family, respectively . Depletion of these factors disturbs not only splicing patterns but also the balance of neoblast self-renewal and differentiation confirming their role in fate regulation. Similarly, a member of the Sm family of proteins, which associate with UsnRNAs forming small nuclear ribonucleoproteins (snRNPs) of the spliceosome, has been implicated in neoblast proliferation .
UsnRNAs are short, highly-structured, non-coding RNAs mediating splice site recognition and catalyzing the splicing reaction. In addition to these essential functions as constituents of the splicing machinery, some UsnRNAs regulate splice site choice and alternative polyadenylation . UsnRNA sequence variants are differentially expressed during development  and U1 snRNA variants in particular, were shown to control the expression of cell fate regulators [10,18]. Except for U6 and U6atac, UsnRNAs are transcribed by RNA polymerase 2 (RNApol2) but, in contrast to mRNAs, they are not polyadenylated. Instead, the 3’-ends of their primary transcripts are formed by a co-transcriptional cleavage reaction catalyzed by the Integrator complex . This 3’-processing step is required for their nuclear export, their association with Sm proteins and, consequently, for their function in splicing. The Integrator complex consists of at least 14 subunits (ints) of which ints9 and ints11 form an endonuclease that cleaves nascent pre-UsnRNAs close to a sequence element termed 3’ box [19,20]. By mediating U7 snRNA maturation, the Integrator complex also indirectly facilitates the 3’-processing of replication-dependent histone mRNAs [21,22]. In addition to this canonical function, the complex or a subset thereof was implicated in enhancer RNA (eRNA) processing, pause-release of transcription and the response to DNA damage (reviewed in ). Given these important functions, it is not surprising that inactivation of ints severely affects development in a range of animal models [24–28].
Here, we analyzed the differential expression and 3’-processing of UsnRNAs, as central components of the mRNA processing machinery, in neoblasts in vivo. We characterized the UsnRNA repertoire of S. mediterranea and identified neoblast-enriched sequence variants. In line with higher expression levels of ints, we found evidence for enhanced UsnRNA 3’-processing in neoblasts compared to differentiated cells. Using RNAi-mediated ints depletion, as a means of disrupting UsnRNA maturation, we analyzed their role in neoblast fate regulation and identified an essential function in the maintenance of the neoblast pool.
Identification of neoblast-enriched planarian UsnRNAs
UsnRNA variants are differentially expressed during development and have been implicated in pluripotency maintenance in vitro [10,17,18]. To analyze their function in adult stem cells in vivo, we first characterized the S. mediterranea UsnRNA repertoire employing a combination of in silico identification of putative UsnRNA genes and validation of their expression by small RNA sequencing. Since UsnRNA secondary structures are evolutionary conserved, we performed a structure-guided similarity search for candidate genes in the S. mediterranea genome [29–31]. This search identified a total of 217 spliceosomal UsnRNA candidates, of which 55 met the statistical threshold (S1 Table). Small RNA sequencing validated the expression of 22 of them. Among these were multiple variants of U1 (11), U2 (2), U4 (3) and U6 (2) snRNA, many of which were present as multiple copies in the genome. Additionally, we identified a putative U4atac candidate, which did not meet the statistical threshold but was validated by RT-PCR (S1 Table, S1A Fig). The predicted secondary structures of the identified UsnRNA types and sequence alignment to their orthologs of other Platyhelminthes species and of Homo sapiens showed substantial conservation of both structural and functional sequence elements, such as the splice site recognition motifs of U1, U5, U6 and U11 snRNA, the branch point recognition motif of U2 and U12 snRNA as well as the sequences mediating the dimerization of U4- and U6-type snRNAs (Fig 1, S1C and S1D Fig, S2 Fig). As expected, the Sm protein binding site (PuA(U)5-6Pu) required for incorporation into snRNP particles is present in all Sm-class UsnRNAs and is highly similar to its vertebrate counterpart [32,33]. Altogether, our analysis identified S. mediterranea orthologs for all spliceosomal UsnRNA types (S1B Fig).
Representative predicted secondary structures of (A) U1 and (B) U2 snRNAs and sequence alignments to their homologs of other Platyhelminthes (Clonorchis sinensis, Echinococcus multilocularis, Schistosoma mansoni and Echinostoma caproni) and Homo sapiens. Primary sequence similarity is indicated in violet and secondary structure conservation (StrucConsensus) is shown below the alignments. Evolutionary conserved structural features and functional sequence elements are indicated: 5’ ss = 5’ splice site recognition motif (green); SL = stem loop; SM = SM protein binding site (red); BPR = branch-point recognition sequence (blue).
To test whether UsnRNA variants are differentially regulated between neoblasts and differentiated cells, we compared their expression levels in intact planarians with those in neoblast-depleted ones, by qRT-PCR. We employed h2b RNAi  and γ-irradiation  as two independent and efficient means of neoblast eradication. Interestingly, while the majority of the identified UsnRNAs were expressed uniformly (S3A Fig), three U1- and one U2 snRNA variants were significantly reduced in both stem cell-depletion conditions (Fig 2A). U1C1, U1C2 and U2A1 showed the most prominent reduction (23–63%), which suggests a substantial enrichment in neoblasts. We corroborated these findings by comparing UsnRNA variant expression between cell populations isolated by fluorescence-activated cell sorting (FACS). Dissociated cells were sorted according to their size and DNA content into a fraction of small cells with high DNA content (>2n) consisting mainly of neoblasts in G2/M phase of the cell cycle (X1 population) and a fraction of larger cells with 2n DNA content, comprised mainly of differentiated cells (Xins population) . Whereas both variants of U1B and U1C snRNA were significantly enriched in the X1 compared to the Xins cell fraction (2–4 fold, Fig 2B), the mild enrichment of U2A1 snRNA in X1 cells (13% higher than in Xins cells, S3B Fig) was not statistically significant. While the predicted secondary structures of the neoblast-enriched variants are overall very similar to those of the uniformly expressed U1A snRNAs (Fig 2C), sequence alignment revealed base changes in both 5’ splice site recognition motif and in loop sequences mediating the interactions with RNPs (Fig 2D). Notably, U1C1 and U1C2, the U1 snRNA variants with the strongest neoblast enrichment, possess an alternative base within the 5’ splice site recognition motif (Fig 2D, S1 Table, S3C Fig) raising the possibility that these UsnRNAs may play a specialized role, perhaps in the recognition of specific splice sites.
(A, B) UsnRNA variant expression, quantified by qRT-PCR, in animals depleted of neoblasts by h2b RNAi (blue) or lethal irradiation (green) and in cell populations isolated by FACS (red). Shown are the variants with significantly changed expression relative to the respective control. Results for other variants are shown in S3 Fig. Expression levels are the averages of three biological replicates, normalized to those of gapdh (error bars represent standard deviation; two-sided t-test, * p<0.05, ** p<0.01, *** p<0.001). (C) Predicted secondary structures and (D) sequence alignment of S. mediterranea U1 snRNA variants. Evolutionary conserved structural features and functional sequence elements are indicated: 5’ ss = 5’ splice site recognition motif (green); SL = stem loop; SM = SM protein binding site (red). Primary sequence similarity is indicated in violet and secondary structure conservation (StrucConsensus) is shown below the alignments.
Disruption of UsnRNA maturation through depletion of Integrator complex subunits
To evaluate the role of the identified UsnRNA variants in planarians, we employed dsRNA-mediated RNA interference (RNAi). However, in contrast to planarian mRNAs , UsnRNAs could not be efficiently depleted using this approach (S4A Fig). As functional maturation of most UsnRNAs requires a 3’ cleavage reaction catalyzed by the Integrator complex [19,38], depletion of Integrator complex subunits (ints) by RNAi should constitute an efficient means for inducing a UsnRNA loss-of-function condition in planarians [19,22]. We performed BLAST searches for ints in the S. mediterranea transcriptome  and identified potential homologs for 12 out of 14 known subunits (S2 Table). For Smed-ints5 and -ints10, homologous candidates with low sequence similarity were identified using the Planmine database BLAST annotation. Based on their BLAST scores and their previous identification in an S. mediterranea stem cell proteome , we focused on Smed-ints3 and -ints9 (ints3 and ints9) for further analysis.
To test for a requirement of the Integrator complex for UsnRNA 3’-processing in planarians, we inhibited ints3 or ints9 expression by RNAi, followed by qRT-PCR quantification of 3’-unprocessed UsnRNAs. To correct for potential effects of ints RNAi on UsnRNA transcription, we normalized the level of the 3’-unprocessed UsnRNA to that of the respective total pool. Importantly, depletion of ints3 or ints9 resulted in a significant accumulation of the unprocessed forms of all tested UsnRNA variants, except for U11 and U12 (Fig 3A). We observed the highest accumulation of the unprocessed form for U2 snRNAs (20- and 35-fold in ints3- and ints9 RNAi animals, respectively). These results show that ints3 and ints9 are functional planarian homologs of Integrator complex subunits and that their depletion disrupts UsnRNA maturation.
qRT-PCR quantification of transcript levels. (A, B) Depletion of Integrator complex subunits results in a significant accumulation of 3’-unprocessed UsnRNAs in both intact animals and in the neoblast-enriched X1 FACS fraction. (C, D) ints3 and ints9 mRNA levels were significantly higher in the X1 fraction compared to both the X2 and the Xins fraction, and were strongly down-regulated in γ-irradiated animals depleted of neoblasts. (E) 3’-unprocessed U2A2 snRNA, relative to the total nascent U2A2 level, was less abundant in RNA of X1 cells compared to that of Xins cells (left chart; average of the X1/Xins ratios of 3 biological replicates; p = 0.02, two-sided heteroscedastic t-test) and accumulated substantially in X1 cells upon ints RNAi (right chart). Expression levels are the averages of three biological replicates normalized to those of the total pool of the respective UsnRNA variant in A+B, to those of gapdh in C+D and to those of total nascent U2A2 in E (error bars represent standard deviation; two-sided t-test, * p<0.05, ** p<0.01, *** p<0.001).
High ints expression and Integrator-mediated UsnRNA processing activity in neoblasts
Next, we employed the UsnRNA processing assays to RNA of X1 cells isolated by FACS from ints RNAi animals. For all UsnRNAs analyzed, we observed even higher average accumulation of the unprocessed forms in ints RNAi X1 cells than for RNA extracted from entire ints RNAi animals. Except for U4A2, all unprocessed forms were significantly increased in either ints9 or ints3 RNAi X1 cells, or both, compared to X1 cells of control RNAi animals (Fig 3B).
The requirement of the Integrator complex for UsnRNA 3’-processing in X1 cells tempted us to analyze the expression of ints in different cell populations. Thus, we compared the mRNA levels of the putative ints homologs in previously published RNA expression data of FACS-isolated cell fractions . Interestingly, all of the putative ints homologs showed higher expression in the X1 FACS fraction than in the Xins fraction, with an average enrichment of 4.8-fold, and five of the subunits had been previously classified as neoblast-enriched  (S3 Table). We confirmed the significantly higher expression of ints3 and ints9 in the X1 compared with the Xins population by qRT-PCR (Fig 3C). Consistently, γ-irradiated animals showed strongly reduced levels of both transcripts (Fig 3D).
Increased mRNA expression of ints may translate into a higher activity of the snRNA 3’-processing machinery. We therefore quantified the levels of 3’-unprocessed UsnRNA in X1 and Xins cell fractions isolated by FACS. To be able to correct for differential snRNA transcription- and degradation rates in the different cell fractions, we developed a qRT-PCR assay that quantifies the total nascent transcript level by detecting a short 3’ extension present in 3’-unprocessed UsnRNA and in an Integrator-processed, short-lived intermediate but not in the mature UsnRNA. We focused on U2A2 snRNA since the Integrator-processed intermediate of its counterpart in other species (U2+10 nt) was reported to have a relatively long 3’-extension [42,43], allowing to distinguish it from the mature form by PCR (Scheme in Fig 3E). Sanger sequencing identified position 187 as the mature end of U2A2 snRNA (S4B Fig) and we employed a reverse primer annealing to positions 165–192 to quantify the total nascent transcript level. After normalizing to this level, we found that the 3’-unprocessed U2A2 snRNA was, on average, 14% less abundant in RNA of the X1 compared to the Xins fraction (p = 0.02, two-sided heteroscedastic t-test), indicating that neoblasts possess not only a distinct composition of spliceosomal UsnRNAs but likely also a higher UsnRNA processing activity than differentiated cells. Importantly, both ints3 and ints9 RNAi resulted in an approximately 3-fold increase in the level of unprocessed U2A2 snRNA in the X1 population (Fig 3E), demonstrating the importance of the Integrator complex for UsnRNA processing in neoblasts.
Neoblasts are progressively lost upon ints depletion
Neoblasts are strictly required for tissue turnover and regeneration in planarians . Hence, an essential role of Integrator complex-mediated UsnRNA processing in neoblast function would be reflected by defects in these processes upon ints depletion. Monitoring intact ints RNAi animals for several weeks, we found that they developed head regression and ventral curling, phenotypes indicative of a neoblast defect , and died after a median survival time of 27 days (ints9 RNAi) or 33 days (ints3 RNAi) after the first dsRNA injection (Fig 4A, S5A Fig). Interestingly, amputated ints RNAi animals initiated regeneration but developed only a very small regeneration blastema and completely failed to regenerate photoreceptors or pharynges (Fig 4B). 7 days following amputation, when 1 or both eyes were already visible in the majority of control trunk and tail fragments, none of the ints RNAi fragments had regenerated eyes (Fig 4C). Like homeostatic RNAi animals, they eventually underwent ventral curling and died approximately 3 weeks after the first RNAi treatment.
(A) Survival curves of homeostatic ints RNAi animals showing median survival times of 32 and 26 days after the first RNAi treatment (days of RNAi) for ints3 and ints9 RNAi, respectively. Corresponding live images are in S4A Fig. (B) Representative live images of ints RNAi fragments 7 days post amputation (7 dpa; 20 days RNAi) displaying significantly reduced blastema size, lack of eye regeneration (white arrowheads) and defective remodeling of existing tissues to form a pharynx (black arrowheads). (C) Quantification of eye regeneration in trunk and tail fragments corresponding to B (n>32; 2 independent experiments). (D) Cytometry analysis of dissociated cells derived from ints and control RNAi animals revealing a progressive loss of the X1 populations in both amputated and intact ints RNAi animals. Shown is the average proportion of X1 cells in all gated cells of 3 biological replicates. (E) FISH and immunostaining of smedwi-1 mRNA and SMEDWI-1 protein, respectively, in trunk fragments at 7 dpa (20 days of RNAi) confirming the ints RNAi-induced loss of neoblasts and their immediate progeny. The proportion of trunk fragments with the depicted phenotype are indicated (2 independent experiments; Scale bar = 100 μm). (F+G) WISH staining- and qRT-PCR time-course (2–5 dpa) showing that the expression of soxP-1, a marker of sigma neoblasts, is strongly reduced in ints RNAi fragments. The expression levels are the averages of three biological replicates normalized to those of gapdh. (two-sided t-test, * p<0.05, ** p<0.01, *** p<0.001). Error bars represent standard deviation.
To test whether ints depletion indeed affects the neoblast population, we performed flow cytometry of cell suspensions prepared from regenerating ints RNAi vs. control RNAi animals and observed a strong reduction of the X1 fraction at 7 days post amputation (dpa; 17 days of RNAi; Fig 4D). Interestingly, while we observed a strong effect of ints RNAi on UsnRNA processing in neoblasts already at 13 days of RNAi (Fig 3B), the proportion of X1 cells at that time-point was still comparable to that of control RNAi animals. This demonstrates that neoblasts are progressively lost during regeneration of ints RNAi animals and suggests that the disruption of UsnRNA processing is an indirect cause of this loss. To exclude that the neoblast loss is merely a consequence of the observed regeneration defect, we performed the same analysis on homeostatic ints RNAi animals, which showed a similar, progressive reduction of the neoblast fraction (Fig 4D). In line with a slower turnover of neoblasts during homeostasis, their proportion decreased at a slower rate in homeostatic compared to regenerating ints RNAi animals.
We next confirmed the observed neoblast loss using Fluorescent In Situ Hybridization (FISH) of smedwi-1 mRNA, the prototypical stem cell marker in planarians , which was strongly diminished in regenerating ints3 and ints9 RNAi animals at 20 days of RNAi (Fig 4E). Immunofluorescence co-staining of the SMEDWI-1 protein, which is maintained in the immediate progeny of neoblasts (smedwi-1-/SMEDWI-1+ cells), revealed that in both ints RNAi conditions these cells were strongly reduced, albeit to a lesser extent than neoblasts, likely as a consequence of defective neoblast maintenance  (Fig 4E). Depletion of a third putative Integrator complex subunit, Smed-ints11, pheno-copied the regeneration defects and neoblast loss observed upon ints3 and ints9 RNAi (S5B Fig).
Recent studies identified neoblast subpopulations with different lineage potential . To determine whether ints depletion affects sigma neoblasts, which are collectively pluripotent and therefore the most potent subtype known, we analyzed the expression of soxP-1, a marker of these cells, by Whole-mount In Situ Hybridization (WISH) and qRT-PCR at different times following amputation. Strikingly, the sigma neoblast population was already substantially reduced at 2 dpa (15 days of RNAi) in both ints RNAi conditions and decreased further during the following days (Fig 4F and 4G; S6 Fig), suggestive of a progressive loss of sigma neoblasts in ints RNAi animals. Together with increased ints expression and lower levels of unprocessed U2A2 snRNA in neoblasts, these results may suggest a function of UsnRNA processing in the maintenance of the neoblast pool.
ints RNAi affects 3’-processing of histone mRNAs and disrupts neoblast splicing patterns
Disruption of UsnRNA maturation may affect neoblast maintenance by altering different UsnRNA-mediated molecular processes. U7 snRNA, for instance, is required for histone mRNA 3’-end processing [21,22] and histone expression was implicated in neoblast maintenance [34,45]. Hence, we tested the effect of ints depletion on planarian histone processing. We designed qRT-PCR assays to quantify the 3’-unprocessed and the total pool of 5 canonical histone transcripts in RNA of ints- and control RNAi animals at 3 dpa (16 days of RNAi). Normalizing to the total transcript levels, we observed a significant accumulation of 3’-unprocessed mRNAs of histones H2A and H4 for both ints RNAi conditions as well as of H3 for ints3 RNAi animals (Fig 5A), indicating that the known function of the Integrator complex in U7 snRNA- and, thus, indirectly in histone mRNA-processing is conserved in planarians.
(A) qRT-PCR quantification showing that the 3’-unprocessed mRNAs of H2A, H3 and H4 accumulate in regenerating ints RNAi animals (3 dpa; 16 days of RNAi). Expression levels are the averages of three biological replicates normalized to those of the total pool of the respective histone (Error bars represent standard deviation; two-sided t-test, * p<0.05, ** p<0.01). (B) Overview of the splicing events significantly changed in neoblasts depleted of ints3, ints9 or in both conditions showing that the exclusion of alternative (Alt.) exons (exon skipping) is the primary splicing defect induced by ints RNAi. PSI = Percent Spliced-In. For details see S4 Table. (C) Two examples of ints RNAi-induced exon skipping events in neoblasts affecting transcripts encoding putative homologs of dspp and espl1, analyzed by RT-PCR. Results for all other RT-PCR validations are shown in S7A Fig. (D) Comparison of the gene expression changes induced by ints RNAi in X1 cells to the transcripts differentially expressed between the X1, X2 and Xin FACS fractions . Transcripts annotated as stem cell-specific were statistically significantly over-represented among the transcripts down-regulated in ints RNAi X1 cells (threshold for differentially expressed genes in ints RNAi neoblasts: padj<0.05; |log2 fold-change| > 1; Statistical test for over-/under-representation: hypergeometric test, * p<0.05, *** p<0.001).
Given the key function of UsnRNAs in splicing, we hypothesized that the ints RNAi-induced loss of neoblasts is, at least in part, due to the disruption of their splicing patterns. To globally characterize the effects of ints depletion on the splicing patterns of neoblasts, we sequenced the transcriptomes of X1 cells isolated from ints3- and ints9 RNAi animals at 3 dpa (16 days of RNAi) and compared their splicing patterns to that of control RNAi X1 cells. As described recently, we quantified the inclusion frequency (PSI; Percent Spliced In) of alternative exons and introns as well as the differential usage of alternative donor and acceptor splice sites [14,47,48]. This analysis identified 295 and 152 splicing events that showed a significant change in inclusion levels (│PSI ints RNAi-PSI ctrl RNAi│>15%, see Methods) in X1 cells of ints3 and ints9 RNAi animals, respectively, compared to their control RNAi counterparts (Fig 5B, S4 Table). Although the total number of splicing changes differed, a strong bias towards exon skipping (exclusion of exons) was observed for both RNAi conditions (68.8% and 51.9% of all events, respectively) and only a very small number of intron retention events were detected (2.7% and 1.3%, respectively). Moreover, a core set of 63 splicing events were affected in the same direction in both RNAi conditions (S4 Table). 14/15 (93%) tested exon skipping events shared by ints3 and ints9 RNAi X1 cells were validated by RT-PCR (Fig 5C, S7A Fig). These results suggest that the defects in UsnRNA maturation induced by ints RNAi affect primarily the exon definition step of splicing in neoblasts. Remarkably, a comprehensive analysis of sequence and structural features of exons skipped upon either RNAi treatment revealed that these are very AT-rich (S8D Fig).
We analyzed the sequencing data of isolated X1 cells further for ints RNAi-induced changes of gene expression, which identified a total of 1351 or 477 transcripts differentially expressed between ints3 or ints9 RNAi and the control, respectively (padj<0.05, │log2 fold-change│>1, S5 Table). Importantly, the overlap between the mis-spliced and the differentially expressed transcripts was minimal (15 and 0 for ints3 and ints9 RNAi, respectively; S7B Fig), suggesting that the majority of the splicing defects did not directly affect mRNA stability. The overlap between ints3 and ints9 RNAi-induced changes in transcript levels, however, was substantial (S7C Fig) indicating that these changes are indeed caused by a loss of Integrator complex function. We next compared these changes to expression data of Labbé et al. , who analyzed the transcriptomes of FACS sorted cell fractions. Interestingly, among the transcripts down-regulated in ints3 or ints9 RNAi X1 cells, we observed a highly significant over-representation of those classified as stem cell-specific (X1) by Labbé et al. (Fig 5D, S5 Table). While these expression changes affected only a subset of all stem cell-specific transcripts, they may indicate a disruption of the normal neoblast gene expression pattern.
Together, these data suggest that defects in histone mRNA processing and splicing collectively contribute to the neoblast loss observed after ints RNAi.
ints RNAi disrupts neoblast self-renewal
We next analyzed the effects of ints depletion on the self-renewal of neoblasts. Since the high density of these cells and of early neoblast progeny complicates their quantification directly within the RNAi animals, we transplanted FACS-isolated X1 cells of ints or control RNAi animals into neoblast-depleted wild-type hosts and analyzed the resulting colonies 5 and 7 days after transplantation (Fig 6A). We identified neoblasts and their differentiating progeny by FISH staining of smedwi-1 and NB.32.1g , respectively, and quantified their proportions. As shown in Fig 6A and 6B, the colonies derived from ints3 and ints9-depleted neoblasts were smaller than those derived from control RNAi neoblasts and contained a significantly higher proportion of differentiating NB.32.1g+ cells (p<0.01 and p<0.001, respectively), demonstrating that ints depletion disrupts the ability of neoblasts to self-renew and changes their fate towards differentiation.
(A) Neoblast transplantation assay. FACS-isolated X1 cells of RNAi animals were transplanted into neoblast-depleted, wild-type hosts. The cell type composition of colonies derived from transplanted neoblasts was analyzed by FISH staining of the neoblast- and the early progeny marker smedwi-1 and NB.32.1g, respectively. ints-depleted neoblasts produced smaller colonies containing an increased proportion of early progeny. Scale bar = 50 μm. dpinj = days post neoblast injection. (B) Quantification of colony composition showing a significant increase in the proportion of early progeny (NB.32.1g+) cells generated from ints-depleted neoblasts. Data of three independent experiments. Circles represent individual colonies. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. n = 46, 50, 51 colonies. Two-sided t-test: ** p<0.01, *** p<0.001.
To test the capacity of ints-depleted neoblasts to differentiate, we pulse-labelled proliferating cells with the thymidine-analog Ethynyl-deoxyuridine (EdU) and analyzed their progeny after different chase periods for the expression of cell type markers representing all germ layers by FISH staining. We normalized the number of marker+EdU+ cells to the total number of EdU+ cells, to account for the substantially lower number of EdU-incorporating neoblasts of ints RNAi animals (Fig 7A). The median numbers of EdU-positive cells expressing early (NB.21.11e+), intermediate (AGAT-1+)  and late (zpuf-6+)  markers associated with the epidermal lineage were substantially lower in ints9-depleted animals compared with controls. A similar reduction was observed for myoD+EdU+ muscle cell progenitors  and collagen+EdU+ muscle cells . The numbers of gata4/5/6+EdU+ gut precursors  and of terminally-differentiated intestinal cells expressing porcupine  were reduced in ints3 RNAi animals. Furthermore, we observed a mild reduction in the number of EdU+ neurons expressing prohormone convertase 2 (pc2)  in both ints RNAi conditions. Importantly, EdU-positive cells expressing markers of late progeny and of terminally differentiated cell types were still observed in ints RNAi animals (Fig 7B–7D). These findings indicate that neoblast progeny is able to differentiate even at very low Integrator complex activity, yet gives rise to a lower number of differentiated cells, possibly due to a reduction in general cell fitness.
EdU pulse-chase labeling combined with FISH staining of differentiation markers. 10 days following the first dsRNA injection, animals were pulsed with EdU, fixed 3, 7 or 9 days later and cell type markers were visualized by FISH. (A) Quantification of EdU-positive cells expressing the indicated marker, normalized to the total number of EdU-positive cells. Chase periods were 3 days for NB.21.11.e, myoD and gata4/5/6, 7 days for AGAT-1 and 9 days for porcupine, pc2, zpuf-6 and collagen. Circles represent measurements of individual animals. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. (B, C, D) Representative images of EdU-labelled cells expressing the intestinal (porcupine), neuronal (pc2) or epidermal progeny (zpuf-6) marker. Overview images and insets are single confocal sections acquired with a 20x and a 63x objective, respectively. Arrowheads indicate marker+EdU+ cells. Scale bars: overview pictures = 50 μm, magnifications = 5 μm.
Here, we describe the UsnRNA repertoire of the planarian S. mediterranea and investigate the impact of disturbed Integrator complex-mediated UsnRNA processing on the fate of its stem cells. We first identified spliceosomal UsnRNAs using a bioinformatics approach that has been successfully employed in other non-mammalian model organisms [30,54,55]. The validation of their expression by small RNA sequencing effectively removed pseudogenes, as shown independently by RT-PCR. While this approach might have excluded some UsnRNA candidates that are expressed at low levels and/or have high sequence similarity to other candidates, the numbers of validated genes encoding the different UsnRNA types are in the range of those predicted for their counterparts in the closely-related Schistosomes [56,57] suggesting that they represent the majority of the S. mediterranea UsnRNA repertoire.
As was recently reported for U1 snRNA in cultured mammalian stem cells [10,18], our analysis showed that neoblasts express higher levels of several U1 snRNA variants when compared to differentiated cell types. The similarity of their predicted structure and the conservation of functional sequence motifs suggests that these variants could function in splicing. Given that neoblasts also express several spliceosome components at higher levels  and that increased spliceosome activity has been linked to the maintenance of pluripotency , we speculate that increased UsnRNA levels support a higher spliceosome activity. Moreover, the modified 5’ splice site recognition motif present in the neoblast-enriched U1C1 and U1C2 snRNA variants indicates that they might facilitate the use of specific 5’ splice sites or, alternatively, act as dominant negative regulators suppressing differentiation-related AS events. A role for these variants in alternative polyadenylation is also plausible . Of note, even minor U1 snRNA variants may exert substantial effects on gene expression .
In FACS-sorted cell fractions enriched for neoblasts, we found lower levels of 3’-unprocessed U2A2 snRNA, relative to the total nascent transcript, than in cell fractions consisting of differentiated cells. This raises the possibility that the 3’-processing of UsnRNAs constitutes a cell type-dependent control mechanism for UsnRNA abundance and hence for UsnRNA-dependent processes, such as splicing. While lower levels of unprocessed UsnRNAs could alternatively be explained by higher degradation rates, the observed increase of unprocessed U2A2 in neoblasts upon inhibition of the UsnRNA 3’-processing machinery supports our hypothesis. Together with our qRT-PCR results and previously published RNAseq data , which show that ints-encoding transcripts are enriched in neoblasts, it is likely that this increased processing activity is, at least in part, due to higher ints expression in these cells.
Testing the function of UsnRNAs directly requires efficient and highly sequence-specific methods for UsnRNA depletion or over-expression, both of which are currently not available in S. mediterranea. In contrast to mRNAs, UsnRNAs could not depleted by dsRNA-mediated RNAi, which is likely due to differences in expression levels, subcellular localization or structure of these RNA types. We therefore used RNAi against Integrator complex subunits as efficient means to disrupt UsnRNA maturation and hence function. This caused a plethora of splicing defects and the accumulation of unprocessed histone mRNAs. The latter is likely a consequence of defective U7 snRNA processing by the Integrator complex, since U7 snRNA is known to mediate the maturation of replication-dependent histone mRNAs [21,22,58]. Given the importance of histone expression for neoblast maintenance , the reduced processing of H2A, H4 and H3 mRNAs is likely to contribute to the neoblast maintenance defect of ints RNAi animals. While splicing defects are an expected consequence of the loss of UsnRNA processing, the very high proportion of exon skipping, but not intron retention, events among the splicing changes observed in both ints RNAi conditions was surprising and suggests that ints depletion impairs primarily exon definition. Interestingly, very similar splicing changes were observed in ints8-mutant mammalian cells , substantiating our results and supporting an evolutionary conserved function of the Integrator complex in facilitating splicing fidelity. As many of the exon skipping events are likely to disrupt the reading frame of the corresponding transcripts, reducing the production of functional proteins and causing the accumulation of potentially cytotoxic proteins, they may interfere with neoblast maintenance. Indeed, depletion of either ints3 or ints9 disrupted tissue homeostasis and regeneration, likely as a consequence of the gradual neoblast loss. While the Integrator complex has not been implicated in stem cell maintenance to date, knock-out of ints genes causes early developmental lethality in diverse animal models that could well be explained by defects of stem cell maintenance [24–27].
Investigating a putative effect of ints RNAi on the transcriptional profile of neoblasts we revealed a considerable de-regulation of gene expression. Whether this was due to defective histone processing or the disruption of a putative transcriptional regulator function of the Integrator complex [60–62] is unclear but the minimal overlap between mis-spliced and differentially expressed transcripts suggest that defective splicing is not a major contributor. Despite a stronger effect of ints9 RNAi on the survival of planarians, we observed a higher number of both mis-spliced and differentially expressed transcripts in ints3- compared to ints9 RNAi neoblasts, which may be due to differences in knock-down kinetics or efficiency. Neoblast-specific transcripts were strongly over-represented among those down-regulated in both ints3 and ints9-depleted neoblasts. It is important to point out that these transcriptomic changes occur in neoblasts and, thus, do not represent changes in cell type composition. However, they might be early indicators of a neoblast fate change. Transplantation of ints RNAi neoblasts into irradiated host animals confirmed the fate change of these cells towards differentiation. Importantly, since the recipient animals in this experiment were not injected with dsRNA, this cell fate change is likely not due to a loss of Integrator complex function in the host tissue, but rather represents a cell-autonomous phenotype of the transplanted cells. In line with the considerably smaller colonies derived from transplanted ints RNAi neoblasts, EdU pulse-chase experiments revealed that the production of a number of progenitor and differentiated cell types is reduced upon ints depletion. Since the number of neoblasts initially labelled with EdU cannot be determined in these experiments, it is unclear whether the reduced proliferation of ints-depleted neoblasts contributes to this lower cell output. Nonetheless, the presence of EdU-labelled pc2+, porcupine+ and zpuf-6+ cells in the brain, the gut and underneath the epidermis of ints-depleted animals, respectively, suggests that the low levels of ints expression remaining after the incomplete knock-down achieved by RNAi allows for differentiation, at least of a portion of the neoblast progeny.
Neoblasts, similar to germ line stem cells in other organisms, are highly dependent on post-transcriptional regulators of mRNA localization, splicing and/or translation [14,15,58,63–66] as well as on factors generating regulatory RNAs [44,67]. Our study now extends this list by the Integrator complex, which appears to have a dual function in the post-transcriptional regulation of transcripts important for neoblasts: By processing UsnRNA 3’-ends, it mediates splicing and histone mRNA maturation, both of which were previously linked to neoblast maintenance [14,15,34,45]. We propose a model (Fig 8) in which the increased expression of U1 snRNA variants together with enhanced, Integrator-mediated UsnRNA maturation facilitate a higher activity and/or modified specificity of neoblast spliceosomes as well as the efficient U7-dependent processing of histone mRNAs. This may contribute to neoblast maintenance by generating a specific set of protein isoforms as well as by enabling the high turnover of mRNAs and proteins in these mitotically active cells.
Neoblasts express higher levels of U1 snRNA variants and have higher UsnRNA 3’-processing activity, likely due to the high expression levels of Integrator complex components. Together with increased expression of spliceosomal proteins , this may result in a modified specificity and enhanced activity of the spliceosome in neoblasts compared to differentiated cell types. In addition, an increased activity of the Integrator complex may allow efficient histone mRNA 3’-processing through U7 snRNA maturation. Together, these features likely contribute to neoblast maintenance by generating a specific set of protein isoforms as well as by enabling the high turnover of mRNAs and proteins in these mitotically active cells.
Materials and methods
Unless specified otherwise, reagents and devices were purchased from Thermo Fisher Scientific and were used according to the manufacturers recommendations.
UsnRNA prediction, structure modelling and classification
UsnRNA multiple sequence alignments annotated with consensus secondary structures were obtained from the Rfam database v12.0 . For each spliceosomal UsnRNA type, a covariance model was built, calibrated and then searched against the genome of the asexual Schmidtea mediterranea biotype (Asxl v1.1)  using the INFERNAL 1.1.1 programs cmbuild, cmcalibrate and cmsearch , respectively, with default settings. The identified candidate sequences were aligned to the respective covariance model using cmalign of INFERNAL and the resulting, predicted secondary structures were visualized using VARNA 3.93 . For U6atac structure prediction, the candidate sequence was extended at the 3’-end by adding 32 nucleotides from the genomic sequence to fit the model length. Multiple sequence alignments generated by cmalign were visualized with Jalview 2.10.4b1 . Phylograms were generated using Clustal Omega . Variants of the same UsnRNA type were clustered according to sequence similarity and the clusters were named in alphabetical order, starting from the one with the highest number of uniquely-mapping RNAseq reads. Within clusters, variants were given consecutive numbers according to best-mapping read count. Sequence logos were generated using WebLogo generator .
Planarian husbandry, RNAi and EdU pulse-chase
Animals of the asexual, clonal Schmidtea mediterranea line BCN-10 (originally provided by E. Salo), were maintained as described  and treated as specified in S7 Table. Double-stranded RNA (dsRNA) was transcribed from PCR amplicons using T7 RNA polymerase as described previously . Control RNAi animals were injected with dsRNA targeting a region of the green fluorescent protein (GFP) coding sequence. For regeneration experiments, animals were amputated pre- and postpharyngeally into head, trunk and tail fragments. Neoblast depletion by h2b RNAi was performed as described previously . For lethal γ-irradiation, animals were exposed to a dose of 60 Gy using a Gammacell 40 Exactor (Nordion). For EdU pulsing, animals were incubated in (2′S)-2′-Deoxy-2′-fluoro-5-ethynyluridine (F-ara-EdU, Sigma Aldrich)  dissolved in planarian artificial medium (PAM) containing 3% (v/v) DMSO for 16 hours. F-ara-EdU concentrations of 0.4, 0.2 and 0.1 mg/ml were used for 3, 7 and 9 day chase, respectively. Animals were maintained in the dark in PAM containing 50 μg/ml gentamycin throughout the chase period and then fixed with formaldehyde , subjected to FISH and then EdU was stained using the EdU DetectPro Imaging Kit 647 (baseclick GmbH) according to the manufacturer’s protocol.
RNA isolation, cDNA synthesis and qRT-PCR
RNA was isolated using the TRIzol or TRIzol LS reagent. RNA for qRT-PCR analysis of UsnRNAs or for mRNA sequencing was treated with TURBO DNase followed by either phenol-chloroform extraction and ethanol precipitation or by purification on NucleoSpin RNA XS columns (Macherey-Nagel). For the latter, RNA was adjusted to a final ethanol concentration of 53% before loading onto the column to improve recovery of small RNA species. First strand cDNA was transcribed using the RevertAid H Minus First Strand cDNA Synthesis Kit with random hexamers (for UsnRNA and histone mRNA assays) or oligo dT primers (for all other mRNA assays), respectively. For the analysis of unprocessed UsnRNA in wild-type X1 and Xins fractions, RNA prepared as described above was subjected to whole transcriptome amplification using the Ovation qPCR system (NuGEN), which was performed with 45 ng of RNA in technical duplicates according to the manufacturers protocol and then pooled. qRT-PCR analysis was performed on the Real-Time PCR System 7500 or 9100 (Applied Biosystems) using either the Universal Probe Library (Roche) together with TaqMan Universal PCR Master Mix or the iTaq universal SYBR Green supermix (Bio-Rad). Primers and probes are summarized in S6 Table. Relative expression levels were calculated as previously described . 3’-unprocessed U2A2 levels were normalized to those of the nascent transcript. End-point RT-PCR was performed in a Mastercycler pro (Eppendorf) using Quick-Load Taq 2X Master Mix (NEB) and analyzed by agarose gel electrophoresis. For S6A Fig, the intensity of the gel bands was quantified using Fiji .
Small RNA sequencing
RNA species of 50–500 nucleotides were isolated from total RNA of intact, wild-type animals by electrophoresis on a 7% polyacrylamide gel containing 7M Urea, extracted from the homogenized gel piece in gel extraction buffer (300mM sodium acetate, 1mM EDTA, 0.01 U/μl SUPERase• In RNase Inhibitor) at 4°C overnight, filtered through a Spin-X centrifuge tube filter (Corning) and concentrated by ethanol precipitation. 500 ng of size-selected RNA was de-capped using RNA 5’ pyrophosphohydrolase (NEB) according to the manufacturers recommendations and then concentrated by ethanol precipitation. The de-capped RNA was then subjected to sequencing library preparation using the NEBNext Small RNA Library Prep Set for Illumina (NEB) according to the manufacturers protocol and analyzed on an Illumina HiScan SQ. Using Bowtie v1.1.2 , the RNAseq reads were mapped onto a compilation of all UsnRNA candidate sequences identified by Infernal cmsearch, each extended by 25 nucleotides on the 3’ end to facilitate the mapping of pre-snRNA-derived reads. To account for the high sequence similarity between variants of the same UsnRNA type, a dual mapping strategy was employed by counting either only uniquely-mapping reads (for validating candidate gene expression) or all best mapping reads (for estimating expression levels). The Bowtie parameters for reporting best- or unique alignments were -a—best -v2 or -a -m1 -v2, respectively. To quantify uniquely-mapping reads, identical candidate sequences in the compilation (colour-coded in S1 Table) were represented by a single sequence. The number of mapped reads were quantified using HTseq-count . For Sanger sequencing (GATC Biotech), individual UsnRNAs were PCR-amplified from the sequencing library described above using a forward primer specific for the respective UsnRNA and the SR RT Primer of the NEBNext Small RNA Library Prep Set for Illumina as the reverse primer (S6 Table).
Flow cytometry was performed as described previously , except that Hoechst 33342 was used at 10 μg/ml and cells were stained at 24°C.
Fluorescence-activated cell sorting (FACS) and cell transplantation
X1-cell isolation was performed on a FACSAria Cell Sorter with its respective software (BD Biosciences) using the protocol described in . Cell transplantations were performed 10 days after the first dsRNA injection as described in , except that approximately 3750 X1 cells in 100 nl were injected per host animal. 5 and 7 days after cell injection, animals were formaldehyde-fixed and processed as described below (S7 Table). For subsequent RNA isolation from cell fractions, cells were stained as described above for flow cytometry and sorted directly into TRIzol LS reagent.
Global analysis of splicing patterns and of differential gene expression
cDNA libraries were prepared from total RNA of the X1 FACS-fraction in biological triplicates using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturers recommendations. Paired-end sequencing (2x75 cycles) was performed on an Illumina NextSeq500. Analysis of alternative splicing was performed using vast-tools v2.0.2. (https://github.com/vastgroup/vast-tools), as recently described [14,47]. Differentially spliced AS events were identified using vast-tools compare, with the following parameters:—min_dPSI 15—min_range 5—noVLOW—p_IR—paired (i.e. │average ΔPSI│>15 and a minimum pairwise difference between paired replicates of | ΔPSI | > 5 in the same direction, excluding events with read coverage score VLOW or lower, and filtering introns that did not pass the binomial test for read balance at the exon-intron junctions). S4 Table contains a summary of the splicing analysis results.
For differential expression (DE) analysis, RNAseq reads were mapped to the reference transcriptome (http://vastdb.crg.eu/libs/vastdb.sme.16.02.18.tar.gz) using vast-tools  to obtain raw read counts. DEseq2  was then used for read count normalization using size factors and pairwise-comparison using contrasts. Genes with padj<0.05 and log2 fold-change ≤ -1 or ≥ 1 were considered significantly differentially expressed. For comparison to previously published RNA expression data of FACS-isolated cell fractions , the corresponding transcripts in the to_Smed_v2 transcriptome were identified by BLAST search and the classification of the highest scoring hit was used for enrichment analysis. The enrichment ratio of class X among DE transcripts was calculated according to: (No. DE transcripts with class X / Total No. DE transcripts) / (No. transcripts with class X in background / Total No. transcripts in background), where background are all transcripts evaluated for DE. The significance of the enrichment was determined using a one-sided hypergeometric test. Venn-diagrams representing overlaps were generated using the VennDiagram package in R .
Immunostaining and In Situ Hybridization of animal whole-mounts
Immunostaining was performed as described previously  with an anti-SMEDWI-1 antibody (1:1000), generated as described in , and Alexa Fluor-conjugated goat-anti-rabbit IgG secondary antibodies (1:400). Chromogenic whole-mount (WISH)- and fluorescent in situ hybridizations (FISH) were performed using the protocols described in [75,84–86] and , respectively. RNA probes were in vitro-transcribed, using T7 or SP6 RNA polymerase, from PCR amplicons generated with the primers listed in S6 Table and labelled with digoxigenin (DIG RNA Labeling Mix, Roche) or dinitrophenol (Label IT Nucleic Acid Labeling Kit, DNP, Mirus Bio). Hoechst 33342 was used at 5 μg/ml as a nuclear counterstain and animals were mounted using Aqua Poly/Mount (Polysciences).
Image acquisition and analysis, Figure preparation
Bands on agarose gels were visualized using a GEL IX 20 (intas). Live animals and WISH stainings were imaged using a Leica M80 and M165 FC microscope, respectively. Confocal microscopy was performed on a Zeiss LSM700 using the ZEN software. CellProfiler  was used for the automated identification and quantification of EdU+ and marker+EdU+ cells. The Classifier functionality of CellProfiler Analyst  was applied to the CellProfiler output to improve identification accuracy for NB.21.11.e, MyoD, gata4/5/6, AGAT-1, zpuf-6 and collagen-positive cells. Images were processed using Fiji  or Adobe Photoshop. Box-Whisker plots were generated using the BoxPlotR web-tool (http://shiny.chemgrid.org/boxplotr/). Figures were prepared in Adobe Illustrator.
Next generation sequencing data is available under Bioproject PRJNA397855: SAMN07490271 (X1_gfp_RNAi), SAMN07490272 (X1_ints3_RNAi), SAMN07490273 (X1_ints9_RNAi), SAMN07490274 (small RNAs_wt_intact). Partial coding sequences of ints were deposited in Genbank: MF671814 (ints3) and MF671815 (ints9).
S1 Fig. Validation, classification and sequence features of UsnRNAs.
(A) Validation of UsnRNA expression. RT-PCR of all tested UsnRNAs produced bands of the expected size (M: molecular weight marker; 100 & 200 bp). Corresponding reactions without reverse transcriptase (-RT), which control for amplification from genomic DNA, had no or a significantly weaker band. (B) Clustering of validated UsnRNAs based on sequence similarity. # indicates the number of identical copies identified in the S. mediterranea genome . (C+D) U4, U4atac and U6atac snRNA predicted secondary structures and sequence alignments to known homologs of other Platyhelminthes (Clonorchis sinensis, Schistosoma mansoni and Echinostoma caproni) and Homo sapiens. Primary sequence similarity is indicated in violet and secondary structure conservation (StrucConsensus) is shown below the alignments. Evolutionary conserved functional sequence elements are indicated: SM = SM protein binding site (red); U4-U6 or U4atac-U6atac base-pairing (yellow).
S2 Fig. Features of Schmidtea mediterranea spliceosomal UsnRNAs.
Representative predicted secondary structures and sequence alignments to their homologs of other Platyhelminthes (Clonorchis sinensis, Echinococcus multilocularis, Schistosoma mansoni and Echinostoma caproni) and Homo sapiens. Primary sequence similarity is indicated in violet and secondary structure conservation (StrucConsensus) is shown below the alignments. Evolutionary conserved functional sequence elements are indicated: ss = splice site recognition motif (green); SM = SM protein binding site (red); BPR = branch-point recognition sequence (blue); UsnRNA base-pairing sequence (yellow).
S3 Fig. Expression of UsnRNAs in neoblast-depleted planarians and FACS cell fractions.
(A+B) UsnRNA variant expression in animals depleted of neoblasts by h2b RNAi (blue), by lethal irradiation (green) and in cell fractions isolated by FACS (red), quantified by qRT-PCR. Expression levels are the averages of three biological replicates, normalized to those of gapdh (two-sided t-test, * p<0.05, ** p<0.01, *** p<0.001). (C) Sequence logos of the 5’ splice site recognition motif of different U1 snRNA variants. The U1 variants with a high average neoblast-enrichment have an alternative base (C → U) annealing to the -1 position of the 5’splice site.
S4 Fig. UsnRNA knock-down and determination of the mature end of U2A2 snRNA.
(A) Injection of dsRNAs targeting U1C or U2A snRNAs did not reduce their expression after 14 days of RNAi. Expression levels are the average of at least 3 biological replicates, normalized to those of gapdh (two-sided t-test, *** p<0.001). (B) Sanger sequence trace showing the 3’ end of U2A2 and the adapter of the RNAseq library from which it was amplified. The mature end is also indicated in the secondary structure model of U2A2 below.
S5 Fig. RNAi phenotypes of ints3, ints9 and ints11.
(A) Live microscopy images showing homeostatic RNAi animals at the indicated times from the first RNAi treatment (days of RNAi). ints RNAi animals developed head regression, ventral curling and eventually lysed. (n = 21, 2 independent experiments). (B) Depletion of the putative ints11 homolog pheno-copied the regeneration and neoblast maintenance defects induced by ints3 and ints9 RNAi. All images and data are from trunk fragments at 8 dpa (n > 10 for live images, 21 d RNAi, 1 experiment). Scale bar = 100 μm.
S6 Fig. qRT-PCR time-course of neoblast subclass markers in amputated ints RNAi animals.
The zeta and sigma neoblast subclass markers were described in . The expression levels are the averages of three biological replicates normalized to those of gapdh. (two-sided t-test, * p<0.05, ** p<0.01, *** p<0.001).
S7 Fig. Validation of splicing defects and overlap of splicing and differential expression data.
(A) Results of the RT-PCR validation of 15 alternative exon splicing events identified by RNAseq of ints RNAi X1 cells. The intensity of the gel bands (see Fig 4B for an example) representing the exon-included and the exon-excluded form were quantified and used to calculate the PSI value. 14 of the 15 events tested were significantly de-regulated in ints3 and ints9 RNAi X1 cells. The events IDs correspond to those in S4 Table (SmeEX(0)nYYYY → ExYYYY). (B, C) Venn-Diagrams showing that the vast majority of the detected splicing defects do not significantly affect the expression of the respective transcript and that a substantial number of gene expression changes are shared by ints3 and ints9 RNAi X1 cells. Thresholds for inclusion are the same as in Fig 5B and 5C.
S8 Fig. Features of exons affected by ints RNAi.
(A, B) Prediction of upstream and downstream splice site strength. (C) Determination of exon length. (D) GC content in affected exons. (E, F) Median length of down- and up-stream introns of affected exons. (G) Statistical significance (p-values) for analyses shown in A-F tested by Mann-Whitney-U-test. AS NC alternatively spliced, but not affected by ints RNAi; CR “cryptic” exons (PSI < 5); CS random subset of 1000 constitutively spliced exons; EX exons with ΔPSI < -15 in ints vs control RNAi; INC exons with ΔPSI > 15 in ints vs control RNAi; bp basepair; ss splice site
S1 Table. S. mediterranea UsnRNAs (Sheet SnRNA_identification) List of UsnRNA gene candidates identified by INFERNAL cmsearch and their RNAseq read counts.
Genes present in multiple, identical copies are marked in the same colour. (Sheet SnRNA_classification) List of all UsnRNA gene candidates with >10 unique reads plus the U4atac candidate. Nomenclature according to sequence similarity and number of best-mapping reads.
S2 Table. Integrator complex homologs in S. mediterranea.
List of putative Smed ints homologs identified by tBLASTx search in the dd_Smed_v6 transcriptome. The sequences of human, mouse and fruitfly ints were used as query. All hits with e-value < 10–5 are shown. N alignments indicates the number of sub-sequences producing hits. For ints5 and ints10, putative homologs were identified from the Planmine blast annotation.
S3 Table. Expression of putative ints homologs in RNAseq data of FACS-sorted cells.
The corresponding IDs in the to_Smed_v2 transcriptome, the respective normalized expression values and the original classification (SC = stem cell, X1-fraction; Prog = Progeny, X2-fraction; Tissues = Xins-fraction) are shown . For ints3, the expression values (FPKM) were obtained from the Planmine annotation of transcript dd_Smed_v6_4426_0_1.
S4 Table. List of ints RNAi-induced splicing changes.
Output of the vast-tools analysis. Column headers: GENE—Gene symbol of mammalian ortholog; EVENT—VAST-DB event ID; COORD—Genomic coordinate of the alternative sequence; LENGTH—Length of the alternative sequence; FullCO—Full set of genomic coordinates of the alternative splicing event; COMPLEX—Type of event; dPSI ints RNAi—Difference of inclusion levels (PSI ints—PSI GFP); X1_GFP_c—Estimated percent of sequence inclusion in sample GFP, Replicate c; X1_GFP_c-Q—Quality scores, and number of corrected inclusion and exclusion reads for sample GFP, Replicate c. The quality scores are encoded as follows: A,B,C,D,E@F,G: A—Read coverage based on actual reads; B—Read coverage based on corrected reads; C—Read coverage, based on uncorrected reads mapping only to the reference C1A, AC2 or C1C2 splice junctions; D—Imbalance of reads mapping to inclusion splice junctions; E—Complexity of the event; F&G—total number of reads, corrected for mappability, supporting inclusion (F) and exclusion (G). For a detailed description see: https://github.com/vastgroup/vast-tools#comparing-psis-between-samples. Sheet Summary: Numbers of identified splicing changes detected in ints RNAi (columns B&C), of those present in the VAST-DB and evaluated for the respective comparison (column D) and enrichment of exon skipping events compared with the respective event type (columns E&F).
S5 Table. DEseq2 analysis of X1-cell RNAseq data.
Normalized read counts, moderated Log2 fold-change and statistical features as reported by DESeq2 (see Methods) for ints3 and ints9 RNAi in comparison to control RNAi. Sheet comparison Labbé et al 2012: Comparison of gene expression changes induced by ints RNAi to the transcripts differentially expressed between the FACS-fractions X1, X2 and Xin as defined in . Up- and down-regulated transcripts relative to control were analyzed separately. P-values were calculated using a one-sided hypergeometric test.
S6 Table. Primers and qRT-PCR probes used in this study.
RNA polymerase promoter sequences are shown in lower case.
We are thankful to Martin Stehling for help with cell sorting, to Suthira Owlarn for critical comments on the manuscript and to all members of the Stem cells & Regeneration Group for fruitful discussions. The Stem Cells & Regeneration Group is part of the Cells in Motion (CiM) Cluster of Excellence.
- 1. Schöler HR, Dressler GR, Balling R, Rohdewohld H, Gruss P. Oct-4: a germline-specific transcription factor mapping to the mouse t-complex. EMBO J. 1990;9: 2185–2195. pmid:2357966
- 2. Rosner MH, Vigano MA, Ozato K, Timmons PM, Poirier F, Rigby PW, et al. A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature. 1990;345: 686–692. pmid:1972777
- 3. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006;126: 663–676. pmid:16904174
- 4. Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O’Hanlon D, Sung H-K, et al. An alternative splicing switch regulates embryonic stem cell pluripotency and reprogramming. Cell. 2011;147: 132–146. pmid:21924763
- 5. Han H, Irimia M, Ross PJ, Sung H-K, Alipanahi B, David L, et al. MBNL proteins repress ES-cell-specific alternative splicing and reprogramming. Nature. 2013;498: 241–245. pmid:23739326
- 6. Chen K, Dai X, Wu J. Alternative splicing: An important mechanism in stem cell biology. World J Stem Cells. 2015;7: 1–10. pmid:25621101
- 7. Fiszbein A, Kornblihtt AR. Alternative splicing switches: Important players in cell differentiation. BioEssays News Rev Mol Cell Dev Biol. 2017;39. pmid:28452057
- 8. Lackford B, Yao C, Charles GM, Weng L, Zheng X, Choi E-A, et al. Fip1 regulates mRNA alternative polyadenylation to promote stem cell self-renewal. EMBO J. 2014;33: 878–889. pmid:24596251
- 9. Frye M, Blanco S. Post-transcriptional modifications in development and stem cells. Dev Camb Engl. 2016;143: 3871–3881. pmid:27803056
- 10. Vazquez-Arango P, Vowles J, Browne C, Hartfield E, Fernandes HJR, Mandefro B, et al. Variant U1 snRNAs are implicated in human pluripotent stem cell maintenance and neuromuscular disease. Nucleic Acids Res. 2016; pmid:27536002
- 11. Kim Y-D, Lee J, Kim H-S, Lee M-O, Son M-Y, Yoo CH, et al. The unique spliceosome signature of human pluripotent stem cells is mediated by SNRPA1, SNRPD1, and PNN. Stem Cell Res. 2017;22: 43–53. pmid:28595116
- 12. Wagner DE, Wang IE, Reddien PW. Clonogenic neoblasts are pluripotent adult stem cells that underlie planarian regeneration. Science. 2011;332: 811–816. pmid:21566185
- 13. Rink JC. Stem cell systems and regeneration in planaria. Dev Genes Evol. 2013;223: 67–84. pmid:23138344
- 14. Solana J, Irimia M, Ayoub S, Orejuela MR, Zywitza V, Jens M, et al. Conserved functional antagonism of CELF and MBNL proteins controls stem cell-specific alternative splicing in planarians. eLife. 2016;5. pmid:27502555
- 15. Fernandéz-Taboada E, Moritz S, Zeuschner D, Stehling M, Schöler HR, Saló E, et al. Smed-SmB, a member of the LSm protein superfamily, is essential for chromatoid body organization and planarian stem cell proliferation. Dev Camb Engl. 2010;137: 1055–1065. pmid:20215344
- 16. Berg MG, Singh LN, Younis I, Liu Q, Pinto AM, Kaida D, et al. U1 snRNP determines mRNA length and regulates isoform expression. Cell. 2012;150: 53–64. pmid:22770214
- 17. Lu Z, Matera AG. Developmental analysis of spliceosomal snRNA isoform expression. G3 Bethesda Md. 2014;5: 103–110. pmid:25416704
- 18. O’Reilly D, Dienstbier M, Cowley SA, Vazquez P, Drozdz M, Taylor S, et al. Differentially expressed, variant U1 snRNAs regulate gene expression in human cells. Genome Res. 2013;23: 281–291. pmid:23070852
- 19. Baillat D, Hakimi M-A, Näär AM, Shilatifard A, Cooch N, Shiekhattar R. Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II. Cell. 2005;123: 265–276. pmid:16239144
- 20. Hernandez N. Formation of the 3’ end of U1 snRNA is directed by a conserved sequence located downstream of the coding region. EMBO J. 1985;4: 1827–1837. pmid:2411548
- 21. Mowry KL, Steitz JA. Identification of the human U7 snRNP as one of several factors involved in the 3’ end maturation of histone premessenger RNA’s. Science. 1987;238: 1682–1687. pmid:2825355
- 22. Ezzeddine N, Chen J, Waltenspiel B, Burch B, Albrecht T, Zhuo M, et al. A subset of Drosophila integrator proteins is essential for efficient U7 snRNA and spliceosomal snRNA 3’-end formation. Mol Cell Biol. 2011;31: 328–341. pmid:21078872
- 23. Rienzo M, Casamassimi A. Integrator complex and transcription regulation: Recent findings and pathophysiology. Biochim Biophys Acta. 2016;1859: 1269–1280. pmid:27427483
- 24. Golling G, Amsterdam A, Sun Z, Antonelli M, Maldonado E, Chen W, et al. Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development. Nat Genet. 2002;31: 135–140. pmid:12006978
- 25. Hata T, Nakayama M. Targeted disruption of the murine large nuclear KIAA1440/Ints1 protein causes growth arrest in early blastocyst stage embryos and eventual apoptotic cell death. Biochim Biophys Acta. 2007;1773: 1039–1051. pmid:17544522
- 26. Rutkowski RJ, Warren WD. Phenotypic analysis of deflated/Ints7 function in Drosophila development. Dev Dyn Off Publ Am Assoc Anat. 2009;238: 1131–1139. pmid:19326441
- 27. Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M, et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature. 2003;421: 231–237. pmid:12529635
- 28. Tao S, Cai Y, Sampath K. The Integrator subunits function in hematopoiesis by modulating Smad/BMP signaling. Dev Camb Engl. 2009;136: 2757–2765. pmid:19605500
- 29. Robb SMC, Gotting K, Ross E, Sánchez Alvarado A. SmedGD 2.0: The Schmidtea mediterranea genome database. Genes N Y N 2000. 2015;53: 535–546. pmid:26138588
- 30. Nawrocki EP, Eddy SR. Infernal 1.1: 100-fold faster RNA homology searches. Bioinforma Oxf Engl. 2013;29: 2933–2935. pmid:24008419
- 31. Nawrocki EP, Burge SW, Bateman A, Daub J, Eberhardt RY, Eddy SR, et al. Rfam 12.0: updates to the RNA families database. Nucleic Acids Res. 2015;43: D130–137. pmid:25392425
- 32. Branlant C, Krol A, Ebel JP, Lazar E, Haendler B, Jacob M. U2 RNA shares a structural domain with U1, U4, and U5 RNAs. EMBO J. 1982;1: 1259–1265. pmid:6202507
- 33. Yong J, Golembe TJ, Battle DJ, Pellizzoni L, Dreyfuss G. snRNAs contain specific SMN-binding domains that are essential for snRNP assembly. Mol Cell Biol. 2004;24: 2747–2756. pmid:15024064
- 34. Solana J, Kao D, Mihaylova Y, Jaber-Hijazi F, Malla S, Wilson R, et al. Defining the molecular profile of planarian pluripotent stem cells using a combinatorial RNAseq, RNA interference and irradiation approach. Genome Biol. 2012;13: R19. pmid:22439894
- 35. Eisenhoffer GT, Kang H, Sánchez Alvarado A. Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea. Cell Stem Cell. 2008;3: 327–339. pmid:18786419
- 36. Hayashi T, Asami M, Higuchi S, Shibata N, Agata K. Isolation of planarian X-ray-sensitive stem cells by fluorescence-activated cell sorting. Dev Growth Differ. 2006;48: 371–380. pmid:16872450
- 37. Sánchez Alvarado A, Newmark PA. Double-stranded RNA specifically disrupts gene expression during planarian regeneration. Proc Natl Acad Sci U S A. 1999;96: 5049–5054. pmid:10220416
- 38. Peart N, Sataluri A, Baillat D, Wagner EJ. Non-mRNA 3’ end formation: how the other half lives. Wiley Interdiscip Rev RNA. 2013;4: 491–506. pmid:23754627
- 39. Brandl H, Moon H, Vila-Farré M, Liu S-Y, Henry I, Rink JC. PlanMine—a mineable resource of planarian biology and biodiversity. Nucleic Acids Res. 2016;44: D764–773. pmid:26578570
- 40. Böser A, Drexler HCA, Reuter H, Schmitz H, Wu G, Schöler HR, et al. SILAC proteomics of planarians identifies Ncoa5 as a conserved component of pluripotent stem cells. Cell Rep. 2013;5: 1142–1155. pmid:24268775
- 41. Labbé RM, Irimia M, Currie KW, Lin A, Zhu SJ, Brown DDR, et al. A comparative transcriptomic analysis reveals conserved features of stem cell pluripotency in planarians and mammals. Stem Cells Dayt Ohio. 2012;30: 1734–1745. pmid:22696458
- 42. Jacobs EY, Ogiwara I, Weiner AM. Role of the C-terminal domain of RNA polymerase II in U2 snRNA transcription and 3’ processing. Mol Cell Biol. 2004;24: 846–855. pmid:14701755
- 43. Westin G, Lund E, Murphy JT, Pettersson U, Dahlberg JE. Human U2 and U1 RNA genes use similar transcription signals. EMBO J. 1984;3: 3295–3301. pmid:6084596
- 44. Reddien PW, Oviedo NJ, Jennings JR, Jenkin JC, Sánchez Alvarado A. SMEDWI-2 is a PIWI-like protein that regulates planarian stem cells. Science. 2005;310: 1327–1330. pmid:16311336
- 45. Guo T, Peters AHFM, Newmark PA. A Bruno-like gene is required for stem cell maintenance in planarians. Dev Cell. 2006;11: 159–169. pmid:16890156
- 46. van Wolfswinkel JC, Wagner DE, Reddien PW. Single-cell analysis reveals functionally distinct classes within the planarian stem cell compartment. Cell Stem Cell. 2014;15: 326–339. pmid:25017721
- 47. Tapial J, Ha KCH, Sterne-Weiler T, Gohr A, Braunschweig U, Hermoso-Pulido A, et al. An atlas of alternative splicing profiles and functional associations reveals new regulatory programs and genes that simultaneously express multiple major isoforms. Genome Res. 2017; pmid:28855263
- 48. Braunschweig U, Barbosa-Morais NL, Pan Q, Nachman EN, Alipanahi B, Gonatopoulos-Pournatzis T, et al. Widespread intron retention in mammals functionally tunes transcriptomes. Genome Res. 2014;24: 1774–1786. pmid:25258385
- 49. Tu KC, Cheng L-C, T K Vu H, Lange JJ, McKinney SA, Seidel CW, et al. Egr-5 is a post-mitotic regulator of planarian epidermal differentiation. eLife. 2015;4: e10501. pmid:26457503
- 50. Cowles MW, Brown DDR, Nisperos SV, Stanley BN, Pearson BJ, Zayas RM. Genome-wide analysis of the bHLH gene family in planarians identifies factors required for adult neurogenesis and neuronal regeneration. Dev Camb Engl. 2013;140: 4691–4702. pmid:24173799
- 51. Witchley JN, Mayer M, Wagner DE, Owen JH, Reddien PW. Muscle cells provide instructions for planarian regeneration. Cell Rep. 2013;4: 633–641. pmid:23954785
- 52. Gurley KA, Rink JC, Sánchez Alvarado A. Beta-catenin defines head versus tail identity during planarian regeneration and homeostasis. Science. 2008;319: 323–327. pmid:18063757
- 53. Agata K, Soejima Y, Kato K, Kobayashi C, Umesono Y, Watanabe K. Structure of the planarian central nervous system (CNS) revealed by neuronal cell markers. Zoolog Sci. 1998;15: 433–440. pmid:18466009
- 54. Chen XS, White WTJ, Collins LJ, Penny D. Computational identification of four spliceosomal snRNAs from the deep-branching eukaryote Giardia intestinalis. PloS One. 2008;3: e3106. pmid:18769729
- 55. Simoes-Barbosa A, Meloni D, Wohlschlegel JA, Konarska MM, Johnson PJ. Spliceosomal snRNAs in the unicellular eukaryote Trichomonas vaginalis are structurally conserved but lack a 5’-cap structure. RNA N Y N. 2008;14: 1617–1631. pmid:18596255
- 56. Marz M, Kirsten T, Stadler PF. Evolution of spliceosomal snRNA genes in metazoan animals. J Mol Evol. 2008;67: 594–607. pmid:19030770
- 57. Copeland CC, Marz M, Rose D, Hertel J, Brindley PJ, Santana C, et al. Homology-based annotation of non-coding RNAs in the genomes of Schistosoma mansoni and Schistosoma japonicum. BMC Genomics. 2009;10: 464. pmid:19814823
- 58. Rouhana L, Weiss JA, King RS, Newmark PA. PIWI homologs mediate histone H4 mRNA localization to planarian chromatoid bodies. Dev Camb Engl. 2014;141: 2592–2601. pmid:24903754
- 59. Oegema R, Baillat D, Schot R, van Unen LM, Brooks A, Kia SK, et al. Human mutations in integrator complex subunits link transcriptome integrity to brain development. PLoS Genet. 2017;13: e1006809. pmid:28542170
- 60. Skaar JR, Ferris AL, Wu X, Saraf A, Khanna KK, Florens L, et al. The Integrator complex controls the termination of transcription at diverse classes of gene targets. Cell Res. 2015;25: 288–305. pmid:25675981
- 61. Gardini A, Baillat D, Cesaroni M, Hu D, Marinis JM, Wagner EJ, et al. Integrator regulates transcriptional initiation and pause release following activation. Mol Cell. 2014;56: 128–139. pmid:25201415
- 62. Lai F, Gardini A, Zhang A, Shiekhattar R. Integrator mediates the biogenesis of enhancer RNAs. Nature. 2015;525: 399–403. pmid:26308897
- 63. Wagner DE, Ho JJ, Reddien PW. Genetic regulators of a pluripotent adult stem cell system in planarians identified by RNAi and clonal analysis. Cell Stem Cell. 2012;10: 299–311. pmid:22385657
- 64. Salvetti A, Rossi L, Lena A, Batistoni R, Deri P, Rainaldi G, et al. DjPum, a homologue of Drosophila Pumilio, is essential to planarian stem cell maintenance. Dev Camb Engl. 2005;132: 1863–1874. pmid:15772127
- 65. Rouhana L, Shibata N, Nishimura O, Agata K. Different requirements for conserved post-transcriptional regulators in planarian regeneration and stem cell maintenance. Dev Biol. 2010;341: 429–443. pmid:20230812
- 66. Solana J, Lasko P, Romero R. Spoltud-1 is a chromatoid body component required for planarian long-term stem cell self-renewal. Dev Biol. 2009;328: 410–421. pmid:19389344
- 67. Palakodeti D, Smielewska M, Lu Y-C, Yeo GW, Graveley BR. The PIWI proteins SMEDWI-2 and SMEDWI-3 are required for stem cell function and piRNA expression in planarians. RNA N Y N. 2008;14: 1174–1186. pmid:18456843
- 68. Darty K, Denise A, Ponty Y. VARNA: Interactive drawing and editing of the RNA secondary structure. Bioinforma Oxf Engl. 2009;25: 1974–1975. pmid:19398448
- 69. Waterhouse AM, Procter JB, Martin DMA, Clamp M, Barton GJ. Jalview Version 2—a multiple sequence alignment editor and analysis workbench. Bioinforma Oxf Engl. 2009;25: 1189–1191. pmid:19151095
- 70. Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li W, et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol. 2011;7: 539. pmid:21988835
- 71. Crooks GE, Hon G, Chandonia J-M, Brenner SE. WebLogo: a sequence logo generator. Genome Res. 2004;14: 1188–1190. pmid:15173120
- 72. Cebrià F, Newmark PA. Planarian homologs of netrin and netrin receptor are required for proper regeneration of the central nervous system and the maintenance of nervous system architecture. Dev Camb Engl. 2005;132: 3691–3703. pmid:16033796
- 73. Sandmann T, Vogg MC, Owlarn S, Boutros M, Bartscherer K. The head-regeneration transcriptome of the planarian Schmidtea mediterranea. Genome Biol. 2011;12: R76. pmid:21846378
- 74. Zhu SJ, Hallows SE, Currie KW, Xu C, Pearson BJ. A mex3 homolog is required for differentiation during planarian stem cell lineage development. eLife. 2015;4. pmid:26114597
- 75. Pearson BJ, Eisenhoffer GT, Gurley KA, Rink JC, Miller DE, Sánchez Alvarado A. Formaldehyde-based whole-mount in situ hybridization method for planarians. Dev Dyn Off Publ Am Assoc Anat. 2009;238: 443–450. pmid:19161223
- 76. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29: e45. pmid:11328886
- 77. Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9: 676–682. pmid:22743772
- 78. Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10: R25. pmid:19261174
- 79. Anders S, Pyl PT, Huber W. HTSeq—a Python framework to work with high-throughput sequencing data. Bioinforma Oxf Engl. 2015;31: 166–169. pmid:25260700
- 80. Moritz S, Stöckle F, Ortmeier C, Schmitz H, Rodríguez-Esteban G, Key G, et al. Heterogeneity of planarian stem cells in the S/G2/M phase. Int J Dev Biol. 2012;56: 117–125. pmid:22450999
- 81. Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014;15: 550. pmid:25516281
- 82. Chen H, Boutros PC. VennDiagram: a package for the generation of highly-customizable Venn and Euler diagrams in R. BMC Bioinformatics. 2011;12: 35. pmid:21269502
- 83. Guo T, Peters AHFM, Newmark PA. A Bruno-like gene is required for stem cell maintenance in planarians. Dev Cell. 2006;11: 159–169. pmid:16890156
- 84. Umesono Y, Watanabe K, Agata K. Distinct structural domains in the planarian brain defined by the expression of evolutionarily conserved homeobox genes. Dev Genes Evol. 1999;209: 31–39. pmid:9914416
- 85. Nogi T, Levin M. Characterization of innexin gene expression and functional roles of gap-junctional communication in planarian regeneration. Dev Biol. 2005;287: 314–335. pmid:16243308
- 86. März M, Seebeck F, Bartscherer K. A Pitx transcription factor controls the establishment and maintenance of the serotonergic lineage in planarians. Dev Camb Engl. 2013;140: 4499–4509. pmid:24131630
- 87. King RS, Newmark PA. In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea. BMC Dev Biol. 2013;13: 8. pmid:23497040
- 88. Lamprecht MR, Sabatini DM, Carpenter AE. CellProfiler: free, versatile software for automated biological image analysis. BioTechniques. 2007;42: 71–75. pmid:17269487
- 89. Jones TR, Carpenter AE, Lamprecht MR, Moffat J, Silver SJ, Grenier JK, et al. Scoring diverse cellular morphologies in image-based screens with iterative feedback and machine learning. Proc Natl Acad Sci U S A. 2009;106: 1826–1831. pmid:19188593