The Integrator complex regulates differential snRNA processing and fate of adult stem cells in the highly regenerative planarian Schmidtea mediterranea
Fig 6
ints depletion disrupts neoblast self-renewal.
(A) Neoblast transplantation assay. FACS-isolated X1 cells of RNAi animals were transplanted into neoblast-depleted, wild-type hosts. The cell type composition of colonies derived from transplanted neoblasts was analyzed by FISH staining of the neoblast- and the early progeny marker smedwi-1 and NB.32.1g, respectively. ints-depleted neoblasts produced smaller colonies containing an increased proportion of early progeny. Scale bar = 50 μm. dpinj = days post neoblast injection. (B) Quantification of colony composition showing a significant increase in the proportion of early progeny (NB.32.1g+) cells generated from ints-depleted neoblasts. Data of three independent experiments. Circles represent individual colonies. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. n = 46, 50, 51 colonies. Two-sided t-test: ** p<0.01, *** p<0.001.