The Integrator complex regulates differential snRNA processing and fate of adult stem cells in the highly regenerative planarian Schmidtea mediterranea
Fig 2
Neoblast-enriched expression of variant U1 snRNAs.
(A, B) UsnRNA variant expression, quantified by qRT-PCR, in animals depleted of neoblasts by h2b RNAi (blue) or lethal irradiation (green) and in cell populations isolated by FACS (red). Shown are the variants with significantly changed expression relative to the respective control. Results for other variants are shown in S3 Fig. Expression levels are the averages of three biological replicates, normalized to those of gapdh (error bars represent standard deviation; two-sided t-test, * p<0.05, ** p<0.01, *** p<0.001). (C) Predicted secondary structures and (D) sequence alignment of S. mediterranea U1 snRNA variants. Evolutionary conserved structural features and functional sequence elements are indicated: 5’ ss = 5’ splice site recognition motif (green); SL = stem loop; SM = SM protein binding site (red). Primary sequence similarity is indicated in violet and secondary structure conservation (StrucConsensus) is shown below the alignments.