Biopsies of human ciliated airway epithelial cell cultures derived from a cystic fibrosis (CF) patient, after CFTR gene delivery, using a novel viral vector that targets human ciliated cells after lumenal delivery. CFTR protein expression (red) was found at the lumenal surface of ciliated epithelial cells where it restored the ability of CF airway cultures to regulate fluid and mucus transport. Ciliated cells transduced by the virus vector are identified with virus-specific antibodies (green). Cell nuclei are in blue. The restoration of CFTR function to CF ciliated epithelial cells fully corrected hallmark features of CF-related pathophysiology in vitro (see Zhang et al., e1000155).
Image Credit: Liqun Zhang, University of North Carolina at Chapel Hill.
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