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Correlative light electron ion microscopy reveals in vivo localisation of bedaquiline in Mycobacterium tuberculosis-infected lungs
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, Fearns et al. developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type-specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, the authors found that the anti-tuberculosis drug bedaquiline is localised not only in foamy macrophages in the lungs during infection but also accumulates in polymorphonuclear cells. The image shows a photomontage in four different pseudo-colors of micro-computed tomography of whole lung from a mouse aerosol-infected with Mycobacterium tuberculosis.
Image Credit: Antony Fearns and Angela Rodgers
Citation: (2021) PLoS Biology Issue Image | Vol. 18(12) January 2021. PLoS Biol 18(12): ev18.i12. https://doi.org/10.1371/image.pbio.v18.i12
Published: January 6, 2021
Copyright: © 2021 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Correlative light, electron, and ion microscopy (CLEIM) offers huge potential to track the intracellular fate of antibiotics, with organelle-level resolution. However, a correlative approach that enables subcellular antibiotic visualisation in pathogen-infected tissue is lacking. Here, Fearns et al. developed correlative light, electron, and ion microscopy in tissue (CLEIMiT) and used it to identify the cell type-specific accumulation of an antibiotic in lung lesions of mice infected with Mycobacterium tuberculosis. Using CLEIMiT, the authors found that the anti-tuberculosis drug bedaquiline is localised not only in foamy macrophages in the lungs during infection but also accumulates in polymorphonuclear cells. The image shows a photomontage in four different pseudo-colors of micro-computed tomography of whole lung from a mouse aerosol-infected with Mycobacterium tuberculosis.
Image Credit: Antony Fearns and Angela Rodgers