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PLoS Biology Issue Image | Vol. 10(8) August 2012

Brain vasculature formation.

The formation of blood vessel networks in the midbrain is captured during development with in vivo long-term serial confocal imaging of intact transgenic zebrafish Tg(kdrl:eGFP), in which vascular endothelial cells express green fluorescent protein. The fate of each vessel segment and endothelial cell is traced in the 3-dimensional vasculature. Besides angiogenic sprouting (filopodium-like process), developing brain vasculature also undergoes extensive blood flow-driven vessel pruning (collapse-like segment), which simplifies the vessel network. This picture represents a projected image of the midbrain vasculature in a larval zebrafish. See Chen et al. (e1001374) in this issue.

Image Credit: Jiulin Du

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Brain vasculature formation.

The formation of blood vessel networks in the midbrain is captured during development with in vivo long-term serial confocal imaging of intact transgenic zebrafish Tg(kdrl:eGFP), in which vascular endothelial cells express green fluorescent protein. The fate of each vessel segment and endothelial cell is traced in the 3-dimensional vasculature. Besides angiogenic sprouting (filopodium-like process), developing brain vasculature also undergoes extensive blood flow-driven vessel pruning (collapse-like segment), which simplifies the vessel network. This picture represents a projected image of the midbrain vasculature in a larval zebrafish. See Chen et al. (e1001374) in this issue.

Image Credit: Jiulin Du

https://doi.org/10.1371/image.pbio.v10.i08.g001