Figures
A colony of stem cells reprogrammed to a pluripotent state from adult brain cells.
2i-iPS cell colony. Inhibitors of the Mek/Erk and GSK3 pathways (2i) in the absence of serum promote the generation of induced pluripotent stem (iPS) cell colonies, shown here. These cells show expression of a pluripotency reporter (green) and reactivation of the silent X chromosome, as demonstrated by the lack of the nuclear red staining body (trimethyl H3K27). For comparison, a cluster of non-reprogrammed cells (non-green) displaying a silent X chromosome (red nuclear body) is shown in the bottom right-hand corner (see Silva et al., e253).
Image Credit: Illustration by Jose Silva and Ornella Barrandon
Citation: (2008) PLoS Biology Issue Image | Vol. 6(10) October 2008. PLoS Biol 6(10): ev06.i10. https://doi.org/10.1371/image.pbio.v06.i10
Published: October 28, 2008
Copyright: © 2008 Silva, Barrandon. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
2i-iPS cell colony. Inhibitors of the Mek/Erk and GSK3 pathways (2i) in the absence of serum promote the generation of induced pluripotent stem (iPS) cell colonies, shown here. These cells show expression of a pluripotency reporter (green) and reactivation of the silent X chromosome, as demonstrated by the lack of the nuclear red staining body (trimethyl H3K27). For comparison, a cluster of non-reprogrammed cells (non-green) displaying a silent X chromosome (red nuclear body) is shown in the bottom right-hand corner (see Silva et al., e253).
Image Credit: Illustration by Jose Silva and Ornella Barrandon