Fig 1.
NIR-GECO evolution and characterization in dissociated neurons.
(a) Mutations of NIR-GECO2 and NIR-GECO2G relative to NIRGECO1. The different mutations between NIR-GECO2 and NIR-GECO2G are highlighted in green. (b) Relative fluorescence intensity (mean ± SEM) of NIR-GECO1, NIR-GECO2, NIR-GECO2G, and miRFP720 in neurons (n = 160, 120, 219, and 84 neurons, respectively, from 2 cultures). Fluorescence was normalized by co-expression of GFP via self-cleavable 2A peptide. (c–e) Comparison of NIR-GECO variants, as a function of stimulus strength (the same color code is used in panels c–e). (c) ΔF/F0; (d) rise time; (e) half decay time. Values are shown as mean ± SD (n = 10 wells from 3 cultures). The underlying data for (b) to (e) can be found in S1 Data. GFP, green fluorescent protein; NIR, near-infrared; SD, standard deviation; SEM, standard error of the mean.
Fig 2.
Performance of NIR-GECO2G, NIR-GECO2, and NIR-GECO1 in HeLa cells.
(a–c) Fluorescence traces of NIR-GECO2G, NIR-GECO2, and NIR-GECO1 in response to 100 ms (a), 500 ms (b), and 1 s (c) blue light activation (470 nm at a power of 1.9 mW/mm2) in HeLa cells with co-expression of Opto-CRAC. Opto-CRAC is composed of the STIM1-CT and LOV2 domain. The fusion of STIM1-CT to the LOV2 domain allows photo-controllable exposure of the active site of SRIMI-CT, which is able to interact with ORAI1 and trigger Ca2+ entries across the plasma membrane [16]. Black, green, and dark blue lines represent averaged data for NIR-GECO2G (n = 32 cells), NIR-GECO2 (n = 25 cells), and NIR-GECO1 (n = 23 cells), respectively. The same color code is used in panels a–c. Shaded areas represent the SD. (d) Quantitative -ΔF/F0 (mean ± SD) for NIR-GECO2G, NIR-GECO2, and NIR-GECO1 in a–c. The underlying data for a–d can be found in S1 Data. NIR, near-infrared; SD, standard deviation.
Fig 3.
Imaging of NIR-GECO2 in response to microfluidic and optogenetic stimulation in C. elegans in vivo.
(a) Left, fluorescent image of neurons expressing NLS-jGCaMP7s (λex = 488-nm laser light, λem = 527/50 nm). Right, fluorescent image of neurons expressing NIR-GECO2-T2A-HO1 (λex = 640-nm laser light, λem = 685/40 nm). Representative of more than 3 worms, both under tag-168 promoter. Scale bar, 50 μm. (b) Fluorescence traces of NLS-jGCaMP7s (top) and NLS-NIR-GECO2 (bottom) in response to the stimulation of microfluidic containing 200 mM NaCl. Solid lines represent averaged data from 3 neurons. Shaded areas are shown as SD. Triangles on the top of the traces indicate the time points of stimulation (20 seconds for each stimulation). (c) Quantitative fluorescence changes of NLS-jGCaMP7s and NLS-NIR-GECO2 in b (n = 36 spikes from 3 neurons). (d) Fluorescence image of the 4 C. elegans expressing NIR-GECO2-T2A-HO1 in AVA neurons (under flp-18 promoter) and CoChR-GFP in ASH neurons (under sra-6 promoter). The merged image is shown. Imaging conditions: NIR-GECO2, λex = 640-nm laser light, λem = 685/40; GFP, λex = 488-nm laser light, λex = 527/50 nm. (e) Individual traces of NIR-GECO2 fluorescence in an AVA neuron under blue light illumination (20 mW/mm2, λex = 488-nm laser light, 100 ms; blue bars). The underlying data for b, c, and e can be found in S1 Data. NIR, near-infrared; SD, standard deviation.
Fig 4.
Comparison of jGCaMP7s and NIR-GECO2 in C. elegans.
(a) Fluorescence intensity of NLS-jGCaMP7s and NIR-GECO2 in C. elegans neurons at resting state. Fluorescence was normalized to the same excitation intensity (n = 132 ROIs from 5 worms; data are shown as mean ± SD) (b) SBR of NLS-jGCaMP7s and NIR-GECO2 in neurons of C. elegans at resting state (n = 132 ROIs from 5 worms; data are shown as mean ± SD). SBR was obtained via dividing the fluorescence intensity from neurons by the averaged autofluorescence from the intestine area. (c) SNR of NLS-jGCaMP7s and NLS-NIR-GECO2 quantified from spontaneously spiking neurons (n = 78 ROIs from 4 worms; data are shown as mean ± SD). SNR was calculated by dividing the fluorescence change associated with a spike by the SD of the baseline fluorescence over the 2-second period immediately before the spike. (d) The ratio of SBRNIR-GECO2 to SBRNLS-jGCaMP7s at different imaging depths (n = 5 worms; data are shown as mean ± SD). (e) The ratio of SNRNLS-NIR-GECO2 to SNRNLS-jGCaMP7s at different imaging depths (n = 4 worms; data are shown as mean ± SD). NIR-GECO2 (without NLS) and NLS-jGCaMP7s were used for the experiments in a, b, and d; NLS-NIR-GECO2 and NLS-jGCaMP7s were used for the experiments in c and e. The underlying data for a–e can be found in S1 Data. ROI, region of interest; SBR, signal-to-background ratio; SD, standard deviation; SNR, signal-to-noise ratio.
Fig 5.
Spontaneous Ca2+ response imaging with NIR-GECO2G in the olfactory bulb of Xenopus laevis.
(a) Light-sheet acquisition of spontaneous Ca2+ response in the olfactory bulb in an intact animal. The image shows an intensity projection over 200 frames and a volume spanning 45 μm. The orientation and position of the field of view is marked in the brightfield image of the animal (upper right frame). Scale bar, 40 μm. (b) White frames indicate the cells that showed Ca2+ response with the NIR-GECO2G (magenta) and GCaMP6s (green) indicator. (c) A representative fluorescence response trace of spontaneous activity of 1 cell in the olfactory bulb (solid white ROI in b) is plotted over time. The intensity responses of NIR-GECO2G and GCaMP6s are antagonal to each other. Z-series volumes were acquired once every 3.0 seconds. NIR, near-infrared; ROI, region of interest.