Fig 1.
Schematic of relationships of major eukaryotic lineages with taxa the subjects of EMS projects indicated with green dots and listed in black text.
The phylogeny is modelled on Keeling and colleagues (2014) [8]. EMS, Experimental Model Systems; sp., species.
Fig 2.
Examples of EMS transformed protists.
(A, B) Corallochytrium limacisporum stably expressing the mCherry fluorescent protein (red) fused to a puromycin resistance protein driven by an endogenous actin promoter (M. Rubio-Brotons, UPF–CSIC, Barcelona, Spain). (C, D) Perkinsus olseni (marine bivalve parasite) expressing a GFP (green) fusion with an exported cell-wall protein (R. Waller, University of Cambridge, United Kingdom). (E, F) The choanoflagellate Salpingoeca rosetta transformed with a plasmid expressing fluorescent proteins that illuminate the cell body (green) and the plasma membrane (magenta) (D. Booth, University of California Berkeley, United States of America). Scale bar = 5 μm, 20 μm, and 5 μm for B, D, and F, respectively. EMS, Experimental Model Systems; GFP, green fluorescent protein; UPF–CSIC, Universitat Pompeu Fabra–Spanish National Research Council.
Table 1.
EMS investigators’ survey summary.
Fig 3.
Global EMS network map of protocols.io PROT-G discussion threads (red) and further direct discussions and interactions (blue) between program teams reported in the EMS survey.
EMS, Experimental Model Systems; PROT-G, Protist Research to Optimize Tools in Genetics.