Figure 1.
Phylogenetic relationships among S. islandicus from the Mutnovsky Volcano.
(A) ClonalFrame [35] reconstruction based on seven loci from 97 S. islandicus strains from the Mutnovsky Volcano region of Kamchatka, Russia (details in Table S1). Strains in purple were isolated from spring M.16. The first number in each name indicates the spring from which strains were isolated, the second indicates the isolate number from that spring, and the third indicates year of isolation. * designates strains selected for genome sequencing and comparison. (B) ClonalFrame phylogeny based on the core genome alignment of 12 S. islandicus strains from hot spring M.16.
Table 1.
Characteristics of 12 strains of S. islandicus from spring M.16.
Figure 2.
Heat map representation of homologous recombination frequency for every donor/recipient pair of branches of the core genome phylogeny of 12 S. islandicus strains.
Recombination frequency is measured relative to its expectation under the prior of the ClonalOrigin model and color coded according to the upper left color/magnitude legend (light blue and blue for the frequency of recombination events below a 1∶1 ratio and yellow to red for the frequency of recombination events above 1∶1). Light gray cells represent non-significant ratios with less than four observed and expected events. White shows number of events that match the prior expectations. Names of strains are color coded as Blue and Red groups.
Figure 3.
Variation in recombination events between the Red and Blue groups through time.
For the Red (A) and Blue (B) recipient strains, the total proportion of events that could be assigned as originating from either donor Red (colored red) and Blue (colored blue) strains is shown as a function of coalescent time with 10 being the most recent divergence and 1 being the common ancestor of this set of strains. A coalescent unit of time is equal to the average length of a generation multiplied by the effective population size.
Figure 4.
FST values between the Red and Blue groups along the chromosome of strain M.16.27.
(A) 10,000 bp windows on the M.16.27 genome where genome sequence is not present in all 10 strains from the Red and Blue groups. These positions highlight variable portions of the M.16.27 genome. *Indicates a recently integrated plasmid. (B) FST values were calculated for sliding windows of 10 kb moving in 5 kb steps. Empty windows where sequence from M.16.27 is not shared by all strains are not plotted. Shading highlights regions of the chromosome that are less differentiated beginning and ending with the first window with FST values lower than 0.5.
Figure 5.
Growth of M.16 strains under standard heterotrophic conditions.
Lines are color coded for strains assigned to the Red and Blue groups. Negative control with no inoculum added is shown in grey. Error bars show the variation in growth among three independent replicate cultures.