Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons
Fig 6
Internal states adjust the Brp compartmental heterogeneity through octopamine.
(A) Graphical summary of the working model. Localized innervation of OANs onto γ KCs regulates the Brp accumulation and forms the Brp compartmental heterogeneity via Octβ2R and cAMP pathway. Nutritional states may modulate OAN activity, thereby adjusting the Brp heterogeneity. (B) Starvation for 48 hours did not affect the Brp::rGFP heterogeneity level in TβhSK2-4 mutant. GFP1−10 was expressed in KCs using R13F02-GAL4 in control, or TβhSK2-4 background flies. 1−2 weeks old flies were used. Pseudo color in the images represents the value of log2 (pixel intensity/mean pixel intensity in the γ lobe). Pseudo color range: −1.2 to 1.2. For the wild-type (WT) group, Fed (n = 16 brains) vs. Starved (n = 17 brains): P = 0.0187; For the TβhSK2-4 group, Fed (n = 16 brains) vs. Starved (n = 17 brains): P > 0.05. Mann–Whitney U-test. (C) Sleep deprivation for 12 h decreases Brp::rGFP heterogeneity level in a Tβh-dependent manner. For the Ctrl group, Undisturbed (n = 13 brains) vs. Sleep deprived (n = 14 brains): P = 0.0222; For the TβhSK2-4 group, Undisturbed (n = 14 brains) vs. Sleep deprived (n = 12 brains): P > 0.05. Mann–Whitney U-test. Pseudo color range: −1.1 to 1.1. Box plots showing center (median), whiskers (Min. to Max.). *P < 0.05 and ns = not significant. The data underlying this Figure can be found in S1 Data.