Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons
Fig 5
Octβ2R and cAMP underlie the Brp compartmental heterogeneity.
(A) Knockdown of Octβ2R reduced the Brp heterogeneity level in γ KCs. Receptors are knocked-down in γ KCs specifically using RNAi with R13F02-GAL4. Kruskal–Wallis test. Ctrl (n = 13 brains) vs. OAMB (n = 11 brains), Octα2R (n = 10 brains) and Octβ2R (n = 12 brains). P = 0.0228 for Ctrl vs. Octβ2R. Ctrl (n = 20 brains) vs. TyrR (n = 22 brains), TyrR2 (n = 22 brains) and OctTyrR (n = 15 brains). *P < 0.05; ns = not significant. (B) Knockdown of Rut significantly decreased the Brp heterogeneity level in γ KCs. Representative images showing Brp::rGFP in control and in Rut knockdown group. Pseudo color in the images represents the value of log2(pixel intensity/mean pixel intensity in the γ lobe). Pseudo color range: −1.2 to 1.2. Mann–Whitney U-test. Ctrl (n = 10 brains) vs. Rut-RNAi (n = 10 brains): P = 0.0011. Box plots showing center (median), whiskers (Min. to Max.). *P < 0.05 and ns = not significant. The data underlying this Figure can be found in S1 Data.