Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons
Fig 4
Octopamine controls the AZ structure in KCs.
(A) OANs in the adult brain. OANs were labeled by Tdc2-GAL4 driven CD4::tdTomato in the left panel. OA-VPM3/4 neurons were labeled with MB022B-GAL4 [10] driven CD8::GFP (right panel). Dashed lines indicate the MB. Scale bars, 20 µm. (B) AZ number and synaptic vesicle localization of OANs along the γ lobe. GFP1–10 and CD4::tdTomato were expressed using Tdc2-GAL4. nSyb::CLIP labeling synaptic vesicles and CD8::GFP were expressed using MB022B-GAL4. Scale bars, 2 µm. Images were cropped from the same MB and are identical in size. (C) AZ density of OANs across γ compartment. Brp::rGFP was visualized using Tdc2-GAL4, and cluster density was quantified. n = 6 brains. Repeated measures one-way ANOVA. γ1 vs. γ2: P < 0.0001; γ1 vs. γ3: P < 0.0001; γ1 vs. γ5: P < 0.0001; γ1 vs. γ5: P < 0.0001. γ2 vs. γ4: P = 0.0098; γ3 vs. γ5: P = 0.0407; γ4 vs. γ5: P = 0.0015. Error bars show S.E.M. (D) Schematic showing the innervation pattern of OANs along the γ lobe. (E) Reduced Brp compartmental heterogeneity level in Tβh mutants. Representative images of Brp::rGFP in control and TβhSK2-4 background are shown. Pseudo color in the images represents the value of log2 (pixel intensity/mean pixel intensity in the γ lobe). Pseudo color range: −1.0 to 1.0. Scale bars, 10 µm. Kruskal–Wallis test. Ctrl (n = 12 brains) vs. TβhSK1-8 (n = 11 brains) and TβhSK2-4 (n = 12 brains). Ctrl vs. TβhSK1-8: P = 0.0442; Ctrl vs. TβhSK2-4: P = 0.0228. *P < 0.05. Box plots showing center (median), whiskers (Min. to Max.). Significant differences (P < 0.05) are indicated by distinct letters or *. The data underlying this Figure can be found in S1 Data.