Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons
Fig 3
Acute starvation decreases the Brp::rGFP heterogeneity level among γcompartments.
(A) Graphical summary of the image analysis. 3D volumes were sampled from different γ compartments. Brp::rGFP clusters are then spatially detected using the 3D spot segmentation Fiji plugin. rGFP signal of individual clusters was measured and the median intensity is calculated for each compartment. (B) Median intensity of Brp::rGFP clusters in γ lobe compartments. R13F02-GAL4 was used. Repeated measures one-way ANOVA. n = 11 brains. γ1 vs. γ2: P < 0.0001; γ1 vs. γ3: P < 0.0001; γ1 vs. γ4: P < 0.0001; γ1 vs. γ5: P < 0.0001. γ2 vs. γ5: P = 0.0101; γ3 vs. γ5: P = 0.0162; γ4 vs. γ5: P = 0.0038. Error bars show S.E.M. (C) Standard deviation (SD) as an indicative of Brp::rGFP heterogeneity level among compartments. The median of Brp::rGFP intensity of clusters in each compartment was log-transformed and SD was calculated from five log-transformed medians for each brain sample. (D) Starvation for 48 hours reduced the Brp heterogeneity level in KCs. One-to-two-week-old flies were used for this experiment. Pseudo color in the images represents the value of log2 (pixel intensity/mean pixel intensity in the γ lobe). Pseudo color range: −1.0 to 1.0. The heterogeneity level (SD) was quantified for individual brain samples. One-way ANOVA on log-transformed data. Fed (n = 19 brains) vs. Starved (n = 17 brains): P = 0.0167; Starved vs. Refeed (n = 19 brains): P = 0.0167; Fed vs. Refeed: P = 0.8271; Scale bar, 10 μm. Box plots showing center (median), whiskers (Min. to Max.). Significant differences (P < 0.05) are indicated by distinct letters. The data underlying this Figure can be found in S1 Data.