Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons
Fig 2
Intracellular active zone heterogeneity among MB compartments.
(A) Brp::rGFP visualized in KCs. Maximum intensity projection showing the horizontal lobe of the MB. Brp::rGFP was visualized using R13F02-GAL4. (B) Diagram showing compartments in the MB γ lobe and the intracellular Brp localization in a single γ KC. (C) Representative maximum intensity projection visualizing Brp::rGFP and CD4::tdTomato in a single γ KC. (D) Signal intensity of Brp::rGFP clusters of single KCs in different compartments. Individual Brp::rGFP clusters are measured. The graph shows median Brp::rGFP intensities of compartments, normalized to the average of the five compartments’ medians. n = 14 KCs (one KC per mushroom body, 9 brains). Repeated measures one-way ANOVA. γ1 vs. γ2: P = 0.0004; γ1 vs. γ3: P < 0.0001; γ1 vs. γ4: P < 0.0001; γ1 vs. γ5: P < 0.0001; γ2 vs. γ3: P < 0.0001; γ2 vs. γ4: P = 0.0001; γ2 vs. γ5: P < 0.0001. (E) Basal Ca2+ concentration near the active zones varies by compartments. The left panel shows the schematic of the Brp::mCherry::GCaMP6s ratiomatric sensor. GCaMP6s sensor is fused to mCherry and Brpshort. The sensor was expressed by R13F02-GAL4 and GCaMP6s signal is normalized by mCherry for analysis. Repeated measures one-way ANOVA. n = 8 brains. γ1 vs. γ3: P = 0.0317; γ1 vs. γ4: P = 0.0307; γ1 vs. γ5: P < 0.0001; γ2 vs. γ3: P = 0.0150; γ2 vs. γ4: P = 0.0307; γ2 vs. γ5: P = 0.0150; Scale bars, 10 μm. Error bars show S.E.M. Significant differences (P < 0.05) are indicated by distinct letters. The data underlying this Figure can be found in S1 Data.