Forty new genomes shed light on sexual reproduction and the origin of tetraploidy in Microsporidia
Fig 8
Binning tetraploid genomes into four subgenomes using BUSCO genes.
(A) Using a greedy algorithm, we iterated through contigs from largest to smallest, appending a contig to a haplotypic subgenome if the duplication contributed by that contig does not exceed a specified threshold (x axes in the figure). Single-copy BUSCO gene completeness is marked by circles and multi-copy BUSCO gene completeness is marked by crosses. A red dashed line denotes the BUSCO completeness score of the unbinned assembly. For (B) idChiSpeb1.µ and (C) ilAceEphe1.µ, we plotted the largest 10 contigs in subgenome 1 with their BUSCO genes, and coloured these genes in the other subgenomes by their positions in subgenome 1. The BUSCO annotations underlying this figure can be found in File Collection 5 at https://doi.org/10.5281/zenodo.17251512. The figure was generated using gerbil (https://github.com/Amjad-Khalaf/gerbil), and manually annotated using InkScape (version 1.2.2).