Plasma membrane remodeling in GM2 gangliosidoses drives synaptic dysfunction
Fig 3
Molecular consequences of GM2 accumulation on the protein composition of the plasma membrane (PM).
A. Quantitative mass spectrometry following enrichment of PM proteins from ΔHEXA and ΔHEXB neurons compared with the SCRM control. A volcano plot is shown with average fold change (x-axis) across three biological replicates and significance (y-axis, two-sided t test) across the three replicates. Targets colored in blue are synaptic proteins and in red are lysosomal proteins with selected targets labelled. B. Select targets are represented graphically to illustrate the fold change in PM protein abundance in ΔHEXA and ΔHEXB neurons vs. SCRM neurons. C. Illustration of proteins with increased abundance at the PM in ΔHEXA and ΔHEXB neurons, compared to SCRM neurons, that play important roles in neuronal signalling such as synaptic vesicle recycling and synaptic adhesion and receptor molecules. Underlying data used to generate these figures are available in S1 Data at https://doi.org/10.17863/CAM.118836.