Single-nucleus transcriptomics of wing sexual dimorphism and scale cell specialization in sulphur butterflies
Fig 9
ChIP-seq profiling of Bab identifies potential target genes involved in the differentiation of Bab– scale types.
A. MEME-ChIP predicted motif for Bab occupancy as inferred from the Bab ChIP pupal wing dataset (top), resembling a previous binding profile for a Bab fly ohnologue (bottom). B. Summary of the position of all predicted Bab ChIP-seq binding sites across two datasets, at the 40% and 60% pupal stages (Data 4-5 at https://osf.io/yjvkc/). Inner circles: HOMER classification of imputed binding sites relative to gene annotation features. Outer rings: overlap with genes that are among the differentially expressed (DE) genes in Scale2 (spatulate) and/or Scale3 (UVI) relative to other scale types. C. Venn diagram featuring the numbers of genes identified as Bab-bound and differentially expressed (DE) in the Scale2-3 clusters, resulting in an intersection of 77 (up) and 53 (down) DE genes with binding sites identified across two stages. D, E, F, H, J. Genomic intervals featuring the Bab ChIP-seq profiles across replicates and peak calls at each stage, including at the promoters of Scale2-3 markers (bottom, buff color). G–G′, I–I″, K-K′. Violin plots of snRNAseq scale cluster expression for the same marker genes.