Low-dose mixtures of dietary nutrients ameliorate behavioral deficits in multiple mouse models of autism
Fig 8
Cocktail supplementation improves the social behaviors of three autism mouse models.
(A, H) Experimental design of cocktail supplementation and behavior assays. OF, open field; EPM, elevated plus maze; 3C, three chamber test; RSI, reciprocal social interaction; CFC, cued fear conditioning. (B-G) The effect of long-term 1/4 cocktail treatment on Tbr1+/− mice. (B) Body weight. (C) Open field. (D) Elevated plus maze. (E) Cued fear conditioning. (F) Reciprocal social interaction. (G) Three-chamber test. (I) The social behaviors of NF1+/−, Cttnbp2+/M120I, and Tbr1+/− mice in the RSI assay were improved by 1/2 cocktail treatment. Data are presented as data points for individual mice and/or means ± SEM. The numbers (n) of examined mice for each group are indicated. (B, I) Paired t test. (C–G) Two-way ANOVA with Bonferroni post hoc test. * P < 0.05, ** P < 0.01, ns = non-significant. Note there is no significant difference in (B–D), though no labeling is indicated. The data underlying the the figure can be found in the S3 Data. All statistical analyses and results, including the actual P-values, are summarized in S4 Data.