Low-dose mixtures of dietary nutrients ameliorate behavioral deficits in multiple mouse models of autism
Fig 7
Mixing low-dose nutrients synergistically improves social behaviors of autism mouse models.
(A, C) Experimental design of dietary supplementation and behavior assays. RSI, reciprocal social interaction. (B) Synergistic effects of the 1/4 cocktail on RSI of WT and Tbr1+/− mice. Individual nutrients, including BCAA (0.45%), serine (1%), and Zn2+ (20 ppm), and the 1/4 cocktail were sequentially applied to mice for 1 week for each supplementation assay. Five RSI tests were conducted as indicated to investigate the effect of supplements on RSI. Left: the response of WT mice in each test; Middle: the response of Tbr1+/− mice in each test; Right: the comparison of WT and Tbr1+/− mice in each test. (D) Synergistic effects of the 1/8 cocktail on Cttnbp2+/M120I mice. Different from (A) and (B), each Cttnbp2+/M120I mouse was only subjected to two RSI tests: one is water control; the other is supplement treatment. Data are presented as data points for individual mice and/or means ± SEM. The numbers (n) of examined mice for each group are indicated. (B) Left and Middle: paired t test was performed to examine the difference between water control and other experimental groups, individually. Right: Two-way ANOVA with Bonferroni post hoc test. (D) Paired t test. * P < 0.05, ** P < 0.01, ns = non-significant. The data underlying the figure can be found in the S3 Data. All statistical analyses and results, including the actual P-values, are summarized in S4 Data.