Low-dose mixtures of dietary nutrients ameliorate behavioral deficits in multiple mouse models of autism
Fig 5
Populations and responses of sociality-linked neurons in the BLA are influenced by Tbr1 deficiency and cocktail supplementation.
(A) Definition of the behavior-related neurons. (B) Representative in vivo calcium imaging traces of behavior-related positively correlated (top), negatively correlated (middle), and irrelevant (bottom) neurons. Left, neuronal activity during the entire session. Right, comparison between the raw cosine similarity (R) and null distribution of shuffled data. (C) Ratio of object exploration- and sociality-linked neurons (n = 300 from four WT mice; n = 242 from four Tbr1+/− mice). (D) Mean activity of object exploration- and sociality-linked neurons during behavior tests. Only positively correlated neurons were analyzed. (E) Connectrograms to reveal repetitively recorded neurons across the four behavioral sessions. (F) Overlap between the water and cocktail experimental groups in terms of object exploration- and sociality-linked neurons. Data in (D) represents mean ± SEM. * P < 0.05, ** P < 0.01; two-way ANOVA with Bonferroni post hoc test. The data underlying the figure can be found in the S3 Data. All statistical analyses and results, including the actual P-values, are summarized in S4 Data.