Low-dose mixtures of dietary nutrients ameliorate behavioral deficits in multiple mouse models of autism
Fig 4
Neural ensembles in the BLA are influenced by Tbr1 haploinsufficiency and cocktail supplementation.
(A) Schematic of the experimental design (WT: n = 4; Tbr1+/−: n = 4). (B) Activity of neurons in the basolateral amygdala (BLA) during object exploration (OE, top) and reciprocal social interaction (RSI, bottom) tests. A set of representative results, including individual neuron activity and mean activity for each neuronal ensemble, is shown. (C) The correlation between the mean neuronal ensemble activity and behavior (binary vector) in the OE (top) and RSI (bottom) tests. (D) Correlation of the activity of all recorded neurons and their corresponding ensembles. The cumulative probabilities of the correlation coefficient of WT and Tbr1+/− mice in OE-1, OE-2, RSI-1, and RSI-2 are shown. (E, F) Activity correlations between dual-recorded neurons and their corresponding neural ensembles. The data points represent the individual neurons. (E) Object exploration, n = 27 from four WT mice; n = 12 from four Tbr1+/− mice. (F) RSI, n = 49 from four WT mice; n = 16 from four Tbr1+/− mice. Data in (C) is mean ± SEM. Statistical analysis in (C) by two-way ANOVA followed by Bonferroni post hoc test. The Kolmogorov–Smirnov test was used for cumulative probabilities in (D). * P < 0.05, ** P < 0.01, *** P < 0.001. See also S5–S7 Figs. The data underlying the figure can be found in the S3 Data. All statistical analyses and results, including the actual P-values, are summarized in S4 Data. The figure was created in BioRender. Lin, M. (2025) https://BioRender.com/cyi56kh.