Low-dose mixtures of dietary nutrients ameliorate behavioral deficits in multiple mouse models of autism
Fig 3
Immunoblotting validation of proteins listed in the Black module.
Four groups of total mouse brain lysates—WT_water, Tbr1+/−_water, WT_1/4 cocktail, and Tbr1+/−_1/4 cocktail––were prepared for immunoblotting. Each group comprises eight mice for analysis. A total of nine antibodies were applied for immunoblotting, as indicated. Quantification was performed by normalization against the total protein levels revealed by Coomassie Blue stain. (A) Representative immunoblots. (B) Quantification of immunoblots. The statistical analysis was conducted using two-way ANOVA and Bonferroni’s post-hoc correction. # P < 0.05; ## P < 0.01; ### P < 0.001 in two-way ANOVA test. *, P < 0.05; **, P < 0.01 in the post-hoc test. Full scans of immunoblots are available in S1 Raw Images. The data underlying the graphs in (B) can be found in the S3 Data. All statistical analyses and results, including the actual P-values, are summarized in S4 Data.