Low-dose mixtures of dietary nutrients ameliorate behavioral deficits in multiple mouse models of autism
Fig 2
Cocktail supplementation alters the total proteome of Tbr1+/− mouse brains.
Four groups of total mouse brain lysates—WT_water, Tbr1+/−_water, WT_1/4 cocktail, and Tbr1+/−_1/4 cocktail—were subjected to LC-MS-MS analysis. The results were subjected to (A) principal component analysis (PCA) and (B–D) Python package for weighted correlation network analysis (PyWGCNA) followed by protein network analysis using STRING. (B) Heatmap of module–trait relationships identified by PyWGCNA. Seven modules and respective numbers of proteins (in brackets) are labeled below the heatmap. In addition to the four groups of samples, the same analyses were performed according to genotype and treatment. In each well, the upper numbers indicate the correlation and the lower numbers in brackets indicate the P value of each analysis. The analysis revealed the highest level of correlation between the “Black” module and the treatment. (C) The Eigengene expression for the Black module. Both the Tbr1+/−_water and WT_water groups display reduced expression compared to the Tbr1+/−_cocktail and WT_cocktail groups. (D) The protein networks and GO for the Black module were analyzed with STRING. The GO terms are color-coded. The corresponding false discovery rates and gene counts are also indicated. See also S1 and S2 Data and S1–S4 Figs.