Species-wide gene editing of a flowering regulator reveals hidden phenotypic variation
Fig 5
Evidence for pleiotropic roles of FLC.
(A) Heatmap of the log2FC values of differentially expressed genes (DEGs) present in at least one contrast. Flowering time data (DTF) from the experiment shown in Fig 1A and 1B. (B) GO enrichment analysis. The top 5 hits (−log(FDR)) are shown with an FDR cutoff of 0.01. (C) Relative expression levels [a.u.] of AZI1 and NRT1.11 in all the wild types and the mutants. AZI1, mean wild types: 0.92 a.u., mean mutants: 0.47 a.u., NRT1.11, mean wild types: 0.47 a.u., mean mutants: 1.37 a.u. (D) Nitrogen isotope composition (δ15N [‰]) in a subset of 29 early flowering mutants and the corresponding wild types (means of three biological replicates). Mean [±se] mutants: 3.17‰ [±0.26]; wild types: 2.43‰ [± 0.46]; Mann–Whitney U rank test, p > 0.05. (E) Difference in δ15N (δ15Ndiff = δ15NWildtype − δ15NMutant) between wild type and the respective mutant. Turquoise indicates significant differences, two-sided Student's t test, Benjamini–Hochberg correction, p.adj. < 0.05. The data underlying this figure can be found in S1 Data and https://doi.org/10.5281/zenodo.15403194.