Species-wide gene editing of a flowering regulator reveals hidden phenotypic variation
Fig 3
Discovery of very early flowering genotypes in Italy.
(A) Comparison of FT phylogeny with flowering time at 16°C (FT16) [39] and FLC expression (FLC normCounts) [42]. The earliest flowering parents of the five F2 populations with a QTL at FT are indicated with an arrow. (B) Early-flowering plants in the wild (at the Angit site). (C) Southern Italian sampling sites. Schematic map on top shows the Angit site. Colors roughly indicate apparent flowering times. (D) Flowering time of progeny from wild plants and control genotypes in the greenhouse (22°C long days). “IDXXXX” is the wild-type strain ID from the 1001 Genomes Project (https://1001genomes.org). The subsequent digits are from a consecutive numbering system for lines selected in the T2 and T3 generations. Similar letters indicate no significant difference in total leaf number (RLN + CLN) (ANOVA with post hoc Tukey HSD, p.adj. < 0.05). The data underlying this figure can be found in S1 Data and https://doi.org/10.5281/zenodo.15403194.