Seizure evolution in a mouse model of West syndrome involves complex and time-dependent synapse remodeling, gliosis and alterations in lipid metabolism
Fig 4
Neuronal and glial changes in the hippocampus of Cyfip2+/R87C mice during seizure evolution.
(A) Representative fluorescence immunohistochemistry images illustrate age-dependent changes in the mean intensities of neuronal (NeuN) and astrocytic (GFAP) markers in the hippocampus of Cyfip2+/R87C mice compared to WT mice. DAPI was used for counterstaining nuclei. High-magnification images of the CA1 region at PWK 28 are shown. SO, stratum oriens, SP, stratum pyramidale, SR, stratum radiatum. (B) Representative fluorescence immunohistochemistry images show age-dependent changes in the mean intensities of oligodendrocytic (Olig2) and microglial (IBA1) markers in the hippocampus of Cyfip2+/R87C mice compared to WT mice. (C) Bar graphs display relative changes in mean intensities of neuronal and glial markers in the hippocampal CA1 region of Cyfip2+/R87C mice compared to age-matched WT mice (n = 7 to 9 mice per genotype, two-way ANOVA with Šídák’s multiple comparisons test). *P < 0.05; **P < 0.01; ***P < 0.001. Data are represented as mean ± standard error of the mean. The data underlying this Figure can be found in S1 Data.