Seizure evolution in a mouse model of West syndrome involves complex and time-dependent synapse remodeling, gliosis and alterations in lipid metabolism
Fig 2
Transcriptomic signatures in the cortex and hippocampus of Cyfip2+/R87C mice during seizure evolution.
(A) Volcano plots display downregulated (blue) and upregulated (red) differentially expressed genes (DEGs) in the cortex and hippocampus of Cyfip2+/R87C mice compared to WT mice at PWK 1, 7, and 14. Bar graphs present significant terms and their corresponding P values from the DAVID (Database for Annotation, Visualization, and Integrated Discovery) Gene Ontology (GO) analysis of the DEGs at PWK 14 (analyses for PWK 1 and 7 were not conducted due to the small number of DEGs). KEGG, Kyoto Encyclopedia of Genes and Genomes. (B) Volcano plots display DEGs in the cortex and hippocampus of Cyfip2+/− mice at PWK 1, 7, and 14. (C) Volcano plots display DEGs in the cortex and hippocampus of Cyfip2+/R87C mice at PWK 28. Bar graphs present significant terms and their corresponding P values from the DAVID GO analysis of the DEGs. (D) Gene Set Enrichment Analysis (GSEA) of the transcriptome from the cortex and hippocampus of Cyfip2+/R87C at PWK 1, 7, 14, and 28, focusing on neuron-, glia-, and other cell-type-specific gene sets. NES, normalized enrichment score, FDR, false discovery rate. (E) GSEA of the transcriptome from the cortex and hippocampus of Cyfip2+/R87C mice, focusing on biological process gene sets, along with clustering of the enriched gene sets using the Cytoscape EnrichmentMap.