Embodiment of an artificial limb in mice
Fig 2
Pupil shifts in the direction of the threatened artificial limb are longer after synchronous stimulation.
(A) Example of vertical (top) and horizontal (bottom) movements of the right pupil during a synchronous (green) and an asynchronous (red) trial for one session. The sequence includes a Baseline, Brush strokes pairing, and a threat to the artificial forelimb. (B) Average vertical (top) and horizontal (bottom) movements of the right pupil during synchronous and asynchronous condition trials, normalized to the average position during the 120 s baseline (n = 10 mice). (C) Schematic of the right side of the mouse face during the experiment. Blue arrow: general direction of the pupillary movement following the threat. (D) Spatial trajectory of the right pupil position from 1 s before to 7 s after the threat starts to be displayed. Top: synchronous pairing. Bottom: asynchronous pairing. (E) Top: Average horizontal movements of the right pupil following the threat onset, normalized relative to the average position 1 s before the threat (n = 10). Light background: SEM. Blue arrow: direction of pupil movement as in C. (F) Average difference between the right pupil movements in the two conditions in E. Blue sections: significant differences (Bootstrap based test p < 0.05). Black dashed line: significance threshold. Gray background: W1 and W2 time windows selected for further quantification. (G) Average values of the profiles displayed in E in the time windows identified in F, for all mice. (H) Same as C–G for the left pupil. The data and code underlying this figure is available in the following repository: https://doi.org/10.5281/zenodo.14635566.