Two distinct subpopulations of human stem-like memory T cells exhibit complementary roles in self-renewal and clonal longevity
Fig 6
The CD95hi subset incorporated more label in vivo compared to CD95int.
(A) Workflow of heavy water labelling technique and timepoints of sample collection. Participants from Cohort 5 drank 70% deuterated water for 7 weeks (100 ml twice daily for one day, then 50 ml twice daily thereafter). Saliva and PBMC were collected at multiple time-points during and after labelling. Cells of interested were sorted and deuterium enrichment was measured by gas chromatography/mass spectrometry (B) M + 1 enrichment of DNA from selected cell populations is shown per individual for all timepoints for the three different subsets. Error bars represent analytic variance and show the standard deviation of 4 replicate measurements. Note, DW19 attained higher levels of label in body water hence the higher enrichment in DNA (the mathematical modelling allows for this variation when calculating cell kinetics). Here lines connect neighboring timepoints for ease of viewing, they do not represent model fits. The data underlying this figure can be found in S1 Data.