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Cuticular collagens mediate cross-kingdom predator–prey interactions between trapping fungi and nematodes

Fig 5

nhr-66 is critical for the fitness and survival of Caenorhabditis elegans in natural environments.

(A) A pie chart showing the number of wild isolates bearing nhr-66 missense variants out of the total number of isolates characterized on CaeNDR. (See S4 Table for more). (B) Nematode escaping rates in wildtype, nhr-66(yph413) mutants, and nhr-66 natural variants (mean ± SEM, n is shown below the x-axis; two-tailed unpaired Student t test). (C) Percentage of nematodes that exploded under hypoosmotic stress (mean ± SEM, n is shown in the figure; two-tailed unpaired Student t test). Right, images of nematodes exposure to deionized water for 15 min (scale bar, 500 μm) (See S2 Video for more). (D) Occupancy rates of wildtype and the nhr-66(yph413) mutant in a soil competition assay at 20 °C (n is shown below the x-axis, two-tailed unpaired Student t test). The data underlying this figure can be found in S5 Data.

Fig 5

doi: https://doi.org/10.1371/journal.pbio.3003178.g005