Cuticular collagens mediate cross-kingdom predator–prey interactions between trapping fungi and nematodes
Fig 2
NHR-66 promotes adhesion through cuticle-secreting tissues without altering cuticle structure.
(A) Quantification of nematode escaping rates in wild-type Caenorhabditis elegans (N2), the nhr-66(yph413) mutant, and tissue-specific rescue lines expressing nhr-66 cDNA under various promoters (mean ± SEM; n is shown below the x-axis; two-tailed unpaired Student t test). (B, C) Atomic Force Microscopy (AFM) topography images (top), quantification and their line profiles (bottom) of adult cuticle annuli, furrows (B) and alae (C) for both wildtype and nhr-66(yph413) mutant. Data are presented as Mean ± SEM (n is shown below the x-axis; two-tailed unpaired Student t test). (D) TEM images and quantification of cuticle (top) and strut width (bottom) from wild-type (N2) and nhr-66(yph413) animals (scale bar: white, 0.2 µm; black, 2 µm). Data are presented as Mean ± SEM (n is shown below the x-axis; two-tailed unpaired Student t test). The data underlying this figure can be found in S2 Data.