Molecular states underlying neuronal cell type development and plasticity in the postnatal whisker cortex
Fig 5
Full-face 10-day all-whisker deprivation (10d AWD) does not influence cell type maturation.
(A) All whisker deprivation is conducted for 10 days, from P12 to P22 (10-d AWD). All whiskers on both sides of the face are plucked, then checked every other day and plucked if there is regrowth. Controls are sham mice, which are anesthetized the same amount of time as 10d AWD but not plucked. (B) Glutamatergic neuronal types have approximately the same relative frequency in P22 10d AWD (y-axis) and normal P22 (x-axis) (S1 Data). (C) PC1 vs. PC2 scatter plot for L2/3 neurons at P22 10d AWD (top) and normal P22 (bottom), highlighting the location of types and the type-specific marker scores. Representation as in Fig 3C. Scores are similar between the two datasets (see also S8D Fig) (S1 Data). (D) L2/3 markers genes, as in S4C Fig, are shown as a function of cells’ position along PC1 comparing patterns between P22 with normal experience and P22 10d AWD (S1 Data).