The m5C reader Ybx1 regulates embryonic cortical neurogenesis by promoting progenitor cell cycle progression
Fig 8
Ybx1 regulates the G1–S phase transition of cortical progenitor cell cycle.
(A) Schematic drawings showing Ybx1 target mRNAs-encoding proteins are involved in regulating normal cell cycle progression. (B and C) Cell cycle flow cytometry analysis determining the percentage of cells in G0/G1, S, and G2/M phases in cortical progenitor cells from E13.5 (B) and E15.5 (C) Ybx1 cKO and littermate control embryos. Data are presented as mean ± SEM: n = 3 replicates; in B, *p = 0.031 for G0/G1 phase, *p = 0.044 for S phase, **p = 0.0012 for G2/M phase; in C, ***p = 4.33E−04 for G0/G1 phase, ***p = 9.75E−04 for S phase, ***p = 3.40E−04 for G2/M phase; by unpaired Student t test. The data underlying all the graphs shown in the figure are included in S1 Data.