Brief early-life motor training induces behavioral changes and alters neuromuscular development in mice
Fig 10
Impact of training on myosin heavy chain subtypes in P3 hindlimb muscles
A. Cross-section through the hindlimb of a P3 mouse labeled by immunofluorescence for laminin (A1). The tibialis anterior is outlined in blue, the lateral gastrocnemius (Gastroc Lat) in orange, and the medial Gastrocnemius (Gastroc Med) in green. Representative images of the immunofluorescence labeling obtained in the tibialis (upper panel) and lateral gastrocnemius muscles (lower panel) for Embryo Myosin heavy chain (MyHC), MyHC type I BAD5 antibody, MyHC IIA SC71 antibody, and MyHC IIB BF-F3 antibody (A2). Calibration bar: 200 μm. B. Violin plots of the muscle area (B1), fiber area (B2), and fiber density (B3) in the three muscles analyzed in untrained (unfilled violins) and trained (purple-filled violins) P3 mice. C. Violin plots of the percentage of MyHC Embryo (C1), MyHC I (C2), and MyHC IIB in the three muscles analyzed in untrained (unfilled violins) and trained (purple-filled violins) P3 mice. Tibialis and gastrocnemius muscles were isolated from 4 trained mice and 5 untrained mice. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-way ANOVA analysis, followed by uncorrected Fisher’s LSD post-tests. Purple asterisks correspond to significant effects associated with training, and black asterisks to significant effects linked to muscle subtype. Underlying data can be found in the S1 Data Sheet.