Brief early-life motor training induces behavioral changes and alters neuromuscular development in mice
Fig 8
Impact of short motor training on axonal myelination in the lumbar spinal cord.
A. Representative fluorescence microscopy image of a transverse spinal cord section depicting fluoromyelin labeling (green channel). Magnified views of the white matter in the dorsal commissure (a, red hatched box), dorsal horn (b, white hatched box), ventral commissure (c and d, green hatched boxes), and ventral horn (e and f, blue hatched boxes) (A1). Representative image of raw data and after the detection of fluoromyelin labeling using Fiji (A2). Calibration bars: 20 μm and 5 μm in magnifications B. Violin plots illustrating the thickness of the myelin sheath (B1) and axon area (B2) in the different regions analyzed for trained (purple filled violins) and untrained (unfilled violins) mice. *p < 0.05, **p < 0.01, ****p < 0.0001, two-way ANOVA analysis, followed by uncorrected Fisher’s LSD post-tests. Graph plot of the myelin sheath thickness as a function of the axon area in trained (purple dots) and untrained (black dots) mice (B3). The lines represent the linear fitting. Four trained and 4 untrained mice were used for this analyze. Underlying data can be found in the S1 Data Sheet.