Brief early-life motor training induces behavioral changes and alters neuromuscular development in mice
Fig 4
Training impact on the Kir and IH currents in P3 lumbar MNs.
A. Representative traces of the current recorded during the application of a long voltage ramp from −40 mV to −150 mV in MNs held at −40 mV in control condition (black trace) and in the presence of 500 μm Ba2+ (blue trace) (A1). The mean cord conductance of the current was measured at membrane potentials equally distant from the reversal potential (Erev), Erev+40 and Erev-40. Violin plots of the chord conductances measured in untrained (black, n = 40) and trained (purple, n = 36) MNs (A2). Violin plots of the chord conductance of the current suppressed by Ba2+ in untrained (black, n = 15) and trained (purple, n = 17) MNs (A3). * Significantly different; *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA analysis, followed by uncorrected Fisher’s LSD post-tests. B. Representative Kir2.2 (green) and GAPDH (red) bands from ventral lumbar spinal cords of untrained and trained P3 mice (top panel), with the quantitative results of western blotting analysis (bottom panel). C. Sample membrane current traces obtained in response to negative voltage steps in a MN held at −60 mV in voltage clamp conditions. The IH current was computed by subtracting the instantaneous current (■) from the steady state (☐) (E1). Mean instantaneous I-V curves derived from the current responses generated by a series of voltage steps in untrained (black dots, n = 14) and trained (purple dots, n = 17) MNs (E2). Underlying data can be found in the S1 Data Sheet.