Brief early-life motor training induces behavioral changes and alters neuromuscular development in mice
Fig 2
Differential gene expression in the LMC of P3 untrained and trained mice.
A. Volcano plot showing RNA-seq data of downregulated (purple open circles) and upregulated (purple filled circles) genes, using a 2-fold change (FC) and a p-value (< 0.05) as selection criteria, indicated by dashed lines. Bar plots showing significant fold changes (log2) in the expression profiles of transcripts encoding transcription factors (B), homeobox and apoptosis-related proteins (C), as well as ion channels, synaptic, and neurotransmission-related proteins (D). Kcna3: potassium voltage-gated channel, shaker-related subfamily, member 3. Kcnc3: potassium voltage gated channel, Shaw-related subfamily, member 3. Kcne3: potassium voltage-gated channel, Isk-related subfamily, gene 3. Kcnh3: potassium voltage-gated channel, subfamily H (eag-related), member 3. Kcng4: potassium voltage-gated channel, subfamily G, member 4. Kcnj12: potassium inwardly-rectifying channel, subfamily J, member 12. Kcnk12: potassium channel, subfamily K, member 12. Kctd12b: potassium channel tetramerization domain containing 12b. Hcn2: hyperpolarization-activated, cyclic nucleotide-gated K+ 2. Scn1b: sodium channel, voltage-gated, type I, beta. Piezo1: piezo-type mechanosensitive ion channel component 1. Pkd2l1: polycystic kidney disease 2-like 1. Trpv4: transient receptor potential cation channel, subfamily V, member 4. Camkv: CaM kinase-like vesicle-associated. Sntb2: syntrophin, basic 2. Syndig1l: synapse differentiation inducing 1 like. Rapsn: receptor-associated protein of the synapse. Grik5: glutamate receptor, ionotropic, kainate 5 (gamma 2). Nos1ap: nitric oxide synthase 1 (neuronal) adaptor protein. Adra1d: adrenergic receptor, alpha 1d. Cckar: cholecystokinin A receptor. Sstr3: somatostatin receptor 3. Trh: thyrotropin releasing hormone. Npy: neuropeptide Y. Gad2: glutamic acid decarboxylase 2. Ascl2: achaete-scute family bHLH transcription factor 2. Atf5: activating transcription factor 5. Bex4: brain expressed X-linked 4. Brf1: BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit. Ddx4: DEAD box helicase 4. E2f1: E2F transcription factor 1. E2f8: E2F transcription factor 8. Foxp2: forkhead box P2. Foxq1: forkhead box Q1. Gdf5: growth differentiation factor 5. Gfra4: glial cell line derived neurotrophic factor family receptor alpha 4. Igfbp2: insulin-like growth factor binding protein 2. Igfbp7: insulin-like growth factor binding protein 7. Klf14: Kruppel-like transcription factor 14. Neurod6: neurogenic differentiation 6. Notch4: notch 4. Nupr1: nuclear protein transcription regulator 1. Reg3b: regenerating islet-derived 3 beta. Rgmb: repulsive guidance molecule family member B. Sp8: trans-acting transcription factor 8. Spdef: SAM pointed domain containing ets transcription factor. Tcfl5: transcription factor-like 5 (basic helix-loop-helix). Tfap2b: transcription factor AP-2 beta. Wif1: Wnt inhibitory factor 1. Barhl1: BarH like homeobox 1. Barhl2: BarH like homeobox 2. Dlx3: distal-less homeobox 3. Hhex: hematopoietically expressed homeobox. Hdx: highly divergent homeobox. Hoxb6: homeobox B6. Hoxb7: homeobox B7. Hoxc5: homeobox C5. Hoxc11: homeobox C11. Hoxd11: homeobox D11. Hopx: HOP homeobox. Lhx5: LIM homeobox protein 5. Otp: orthopedia homeobox. Vsx2: visual system homeobox 2. Aven: apoptosis, caspase activation inhibitor. Casp6: caspase 6. Pawr: PRKC, apoptosis, WT1, regulator. Raw data are available under the following link: https://doi.org/10.57745/WE0VK8.