Polarized subcellular activation of Rho proteins by specific ROPGEFs drives pollen germination in Arabidopsis thaliana
Fig 2
GEF8, GEF9, and GEF12 are necessary for pollen germination and male fertility.
(A) Pollen germination efficiency of in vitro germinated pollen 4 h after imbibition on pollen germination media. Each point represents one replicate with more than 150 pollen grains. Groups of statistically significant differences according to a one-way ANOVA with Tukey’s test (p < 0.05) are indicated with letters. (B) Representative images of in vitro germinated pollen 4 h after imbibition on PGM. (C) Quantification of mutant allele frequency in F1 generation of reciprocal crosses with Col-0 as female and the indicated mutants as pollen donors. The heterozygous allele of each genotype is indicated in bold. Asterisks indicate a significant difference in the allele frequency from the expected 50% according to Χ2-test (p < 0.05). For underlying data of all quantification see S1 Data.