Identification of a novel lipoic acid biosynthesis pathway reveals the complex evolution of lipoate assembly in prokaryotes
Fig 5
Phylogeny for clade 3 lipoate:protein ligases without LipM and cpLpl(BA).
LipMs do not have LplB domains and their sequences are consequently shorter. If a sequence is incomplete, parts of the information used to calculate the phylogenetic tree are missing. This can lead to erroneous estimates of the relationships between sequences and can bias the result and weakens statistical significance of the calculation. In addition, Lpl(BA) clearly shows an individual evolution and may also cause weakening of statistical support. The data underlying this figure is provided in S3 Data.