Glycerol suppresses glucose consumption in trypanosomes through metabolic contest
Fig 5
Glycerol metabolism in other trypanosomatids.
(A) Glycerol kinase (GK) and hexokinase (HK) activity in total cell extracts of the procyclic form (PCF) of T. congolense in the presence of glucose (Glc), glycerol (Glyc) or equal amounts of glucose and glycerol (Glc/Glyc). (B) Consumption of glucose (left) and glycerol (right) by the T. congolense PCF incubated in glucose-rich (2 mM), glycerol-rich (2 mM) and glucose/glycerol (2 mM each) conditions. (C) Correlation between high GK/HK activity ratio and preference for glycerol over glucose. BSF, bloodstream form. aHK activity in the presence of equimolar amounts of glucose and glycerol. bRatio between GK and HK activity. cRate of glucose (Glc) or glycerol (Glyc) consumption. dCulture in the presence of 2 mM glucose (+Glc), 2 mM glycerol (+Glyc) or both (+Glc, +Glyc). eData from Fig 1. fND, not detectable. gData from (B). hData from [20]. Data supporting the results described in this figure can be found at https://zenodo.org/record/5075637#.YORd2B069yA.