Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies
Fig 4
Structure of Fab ab1 bound to the N501Y mutant spike protein trimer.
(A and B) Atomic models for the 2 predominant conformations of the spike protein (gray) observed with Fab ab1 (blue) bound to either 2 (A) or 3 (B) receptor binding domains (RBDs) in the up position. (C) Cryo-electron microscopy density map after local refinement with fitted coordinates for the contact zone between the RBD and Fab ab1. (D) Density map in the region near 501 for the N501Y mutant spike protein ectodomain showing density for residues Q498, Y501, and Y505 in the spike protein and a loop in Fab ab1 that includes S30, the residue closest to Y501. (E and F) Close-up views of the contact zone between the RBD region and ACE2 highlighting residues involved.