Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies
Fig 3
Structure of VH ab8 bound to the N501Y mutant spike protein trimer.
(A) Atomic model for the structure of the complex of VH ab8 (blue) with the N501Y mutant spike protein ectodomain (gray). The structure has 2 receptor binding domains (RBDs) in the down position with well-resolved densities for the bound VH ab8. The third RBD is in the up position. (B) Cryo-electron microscopy density map after local refinement with fitted coordinates for the contact zone between the RBD and VH ab8. (C) Density map in the region near 501 for the N501Y mutant spike protein ectodomain showing density for residues Q498, Y501, and Y505. (D and E) Close-up views of the contact zone between the RBD region and ACE2 highlighting residues involved.