Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies
Fig 2
Analysis of VH Fc ab8 and IgG ab1 interactions with N501Y and unmutated spike.
(A) ELISA analysis of antibody interactions with either N501Y or unmutated spike ectodomain. (B) N501Y or unmutated SARS-CoV-2 S pseudotyped virus neutralization by either VH Fc ab8 or IgG ab1. (C and D) ELISA analysis of N501Y or unmutated SARS-CoV-2 spike ectodomain binding by soluble ACE2-mFc in the presence of serial dilutions of either (C) IgG ab1 or (D) VH Fc ab8. ELISA experiments were done at least in duplicate while neutralization experiments were performed twice at least in duplicate, and the average values are shown. Error bars denote the standard error of the mean (SEM).